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> home > Products > Primary Antibodies > Antibody Collections > Catalog Updated > CLCN2 Antibody (N-term)
CLCN2 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P51788 |
| Other Accession | P35525, P51789, NP_001164559.1 |
| Reactivity | Human, Mouse |
| Predicted | Rabbit, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 98535 Da |
| Antigen Region | 209-237 aa |
| Gene ID | 1181 |
|---|---|
| Other Names | Chloride channel protein 2, ClC-2, CLCN2 |
| Target/Specificity | This CLCN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-237 amino acids from the N-terminal region of human CLCN2. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CLCN2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CLCN2 {ECO:0000303|PubMed:29403011, ECO:0000312|HGNC:HGNC:2020} |
|---|---|
| Function | Voltage-gated and osmosensitive chloride channel. Forms a homodimeric channel where each subunit has its own ion conduction pathway. Conducts double-barreled currents controlled by two types of gates, two fast glutamate gates that control each subunit independently and a slow common gate that opens and shuts off both subunits simultaneously. Displays inward rectification currents activated upon membrane hyperpolarization and extracellular hypotonicity (PubMed:16155254, PubMed:17567819, PubMed:19191339, PubMed:23632988, PubMed:29403011, PubMed:29403012, PubMed:36964785, PubMed:38345841). Contributes to chloride conductance involved in neuron excitability. In hippocampal neurons, generates a significant part of resting membrane conductance and provides an additional chloride efflux pathway to prevent chloride accumulation in dendrites upon GABA receptor activation. In glia, associates with the auxiliary subunit HEPACAM/GlialCAM at astrocytic processes and myelinated fiber tracts where it may regulate transcellular chloride flux buffering extracellular chloride and potassium concentrations (PubMed:19191339, PubMed:22405205, PubMed:23707145). Regulates aldosterone production in adrenal glands. The opening of CLCN2 channels at hyperpolarized membrane potentials in the glomerulosa causes cell membrane depolarization, activation of voltage-gated calcium channels and increased expression of aldosterone synthase, the rate-limiting enzyme for aldosterone biosynthesis (PubMed:29403011, PubMed:29403012). Contributes to chloride conductance in retinal pigment epithelium involved in phagocytosis of shed photoreceptor outer segments and photoreceptor renewal (PubMed:36964785). Conducts chloride currents at the basolateral membrane of epithelial cells with a role in chloride reabsorption rather than secretion (By similarity) (PubMed:16155254). Permeable to small monovalent anions with chloride > thiocyanate > bromide > nitrate > iodide ion selectivity (By similarity) (PubMed:29403012). |
| Cellular Location | Cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Cell projection, dendritic spine membrane {ECO:0000250|UniProtKB:P35525}; Multi-pass membrane protein. Cell projection, axon {ECO:0000250|UniProtKB:P35525} Note=Sorting to the basolateral membrane is mediated by AP-1 clathrin adapter (PubMed:16155254). Localizes at axon initial segments and dendritic shaft and spikes. Colocalizes with HEPACAM and GFAP at astrocyte end-foot in contact with brain capillaries and other glial cells (By similarity) (PubMed:22405205, PubMed:23707145) {ECO:0000250|UniProtKB:P35525, ECO:0000250|UniProtKB:Q9R0A1, ECO:0000269|PubMed:16155254, ECO:0000269|PubMed:22405205, ECO:0000269|PubMed:23707145} |
| Tissue Location | Ubiquitously expressed. Moderately expressed in aortic and coronary vascular smooth muscle cells and expressed at a low level in aortic endothelial cells. Expressed in the adrenal gland, predominantly in the zona glomerulosa (PubMed:29403011). Expressed in white mater perivascular astrocytes and ependymal cells (at protein level). |
Research Areas
Citations (0)
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
The transmembrane protein encoded by this gene is a voltage-gated chloride channel that maintains chloride ion homeostasis in various cells. Defects in this gene may be a cause of certain epilepsies. Four transcript variants encoding different isoforms have been found for this gene.
References
Scheper, G.C., et al. Genet Test Mol Biomarkers 14(2):255-257(2010)
Cornejo, I., et al. J. Cell. Physiol. 221(3):650-657(2009)
Thompson, C.H., et al. J. Biol. Chem. 284(38):26051-26062(2009)
Kleefuss-Lie, A., et al. Nat. Genet. 41(9):954-955(2009)
Klaus, F., et al. Biochem. Biophys. Res. Commun. 381(3):407-411(2009)
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$ 192.50
$ 75.00
Cat# AP16730a
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