ARHGEF5 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q12774 |
Other Accession | NP_005426.2 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 176799 Da |
Antigen Region | 1503-1530 aa |
Gene ID | 7984 |
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Other Names | Rho guanine nucleotide exchange factor 5, Ephexin-3, Guanine nucleotide regulatory protein TIM, Oncogene TIM, Transforming immortalized mammary oncogene, p60 TIM, ARHGEF5, TIM |
Target/Specificity | This ARHGEF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1503-1530 amino acids from the C-terminal region of human ARHGEF5. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | ARHGEF5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ARHGEF5 |
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Synonyms | TIM |
Function | Guanine nucleotide exchange factor which activates Rho GTPases (PubMed:15601624). Strongly activates RHOA (PubMed:15601624). Also strongly activates RHOB, weakly activates RHOC and RHOG and shows no effect on RHOD, RHOV, RHOQ or RAC1 (By similarity). Involved in regulation of cell shape and actin cytoskeletal organization (PubMed:15601624). Plays a role in actin organization by generating a loss of actin stress fibers and the formation of membrane ruffles and filopodia (PubMed:14662653). Required for SRC-induced podosome formation (By similarity). Involved in positive regulation of immature dendritic cell migration (By similarity). |
Cellular Location | Cytoplasm. Nucleus Cell projection, podosome {ECO:0000250|UniProtKB:E9Q7D5} |
Tissue Location | Ubiquitously expressed with highest levels in placenta. High levels are also found in colon, kidney, trachea, prostate, liver, pancreas, pituitary gland, thyroid gland and mammary gland. In fetal tissues, expressed at high levels in kidney, lung and liver (PubMed:15601624). Expressed at low levels in lung and heart (PubMed:14662653). |
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Provided below are standard protocols that you may find useful for product applications.
Background
Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization.
References
Bailey, S.D., et al. Diabetes Care (2010) In press :
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Wang, Z., et al. J. Biol. Chem. 284(42):28599-28606(2009)
Lin, Y.M., et al. Mol. Carcinog. 47(12):925-933(2008)
Zhang, Y., et al. Mol. Cell Proteomics 4(9):1240-1250(2005)
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