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PI4K2A Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9BTU6 |
| Other Accession | Q99M64, Q6PE18, NP_060895.1 |
| Reactivity | Human |
| Predicted | Zebrafish, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 54022 Da |
| Antigen Region | 387-415 aa |
| Gene ID | 55361 |
|---|---|
| Other Names | Phosphatidylinositol 4-kinase type 2-alpha, Phosphatidylinositol 4-kinase type II-alpha, PI4K2A |
| Target/Specificity | This PI4K2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 387-415 amino acids from the C-terminal region of human PI4K2A. |
| Dilution | WB~~1:2000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PI4K2A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PI4K2A |
|---|---|
| Function | Membrane-bound phosphatidylinositol-4 kinase (PI4-kinase) that catalyzes the phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4-phosphate (PI4P), a lipid that plays important roles in endocytosis, Golgi function, protein sorting and membrane trafficking and is required for prolonged survival of neurons. Besides, phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4- phosphate (PI4P) is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). |
| Cellular Location | Golgi apparatus, trans-Golgi network membrane; Lipid-anchor. Membrane raft. Cell projection, dendrite {ECO:0000250|UniProtKB:Q2TBE6}. Presynaptic cell membrane {ECO:0000250|UniProtKB:Q2TBE6}. Synapse, synaptosome {ECO:0000250|UniProtKB:Q2TBE6}. Mitochondrion {ECO:0000250|UniProtKB:Q2TBE6}. Endosome. Cytoplasmic vesicle. Membrane; Lipid-anchor. Cell membrane. Perikaryon {ECO:0000250|UniProtKB:Q2TBE6}. Cell projection, neuron projection {ECO:0000250|UniProtKB:Q2TBE6}. Note=Found in subdomains of the plasma membrane termed non-caveolar membrane rafts. Transported from neuronal cell body to neuron projections and neurite tips in a BLOC-1- and AP-3- complexes-dependent manner. {ECO:0000250|UniProtKB:Q2TBE6} |
| Tissue Location | Widely expressed. Highest expression is observed in kidney, brain, heart, skeletal muscle, and placenta and lowest expression is observed in colon, thymus, and small intestine |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs (Barylko et al., 2001 [PubMed 11244087]).
References
Li, J., et al. Oncogene 29(17):2550-2559(2010)
Qin, Y., et al. J. Biol. Chem. 284(34):22544-22548(2009)
Salazar, G., et al. J. Biol. Chem. 284(3):1790-1802(2009)
Craige, B., et al. Mol. Biol. Cell 19(4):1415-1426(2008)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
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