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RAPGEF5 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q92565 |
| Other Accession | NP_036426.3 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 67733 Da |
| Antigen Region | 529-555 aa |
| Gene ID | 9771 |
|---|---|
| Other Names | Rap guanine nucleotide exchange factor 5, Guanine nucleotide exchange factor for Rap1, M-Ras-regulated Rap GEF, MR-GEF, Related to Epac, Repac, RAPGEF5, GFR, KIAA0277, MRGEF |
| Target/Specificity | This RAPGEF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 529-555 amino acids from the C-terminal region of human RAPGEF5. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | RAPGEF5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RAPGEF5 |
|---|---|
| Synonyms | GFR, KIAA0277, MRGEF |
| Function | Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation. |
| Cellular Location | Nucleus. |
| Tissue Location | Widely expressed with highest levels in brain. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF5, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Okada, Y., et al. Hum. Mol. Genet. 19(11):2303-2312(2010)
Hwang, S.J., et al. BMC Med. Genet. 8 SUPPL 1, S10 (2007) :
Rebhun, J.F., et al. J. Biol. Chem. 275(45):34901-34908(2000)
de Rooij, J., et al. J. Biol. Chem. 275(27):20829-20836(2000)
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