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ACACA Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q13085 |
| Other Accession | P11497, Q5SWU9, Q9TTS3, NP_942133.1, NP_942131.1, Q28559 |
| Reactivity | Human |
| Predicted | Bovine, Mouse, Rat, Sheep |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 265554 Da |
| Antigen Region | 2010-2039 aa |
| Gene ID | 31 |
|---|---|
| Other Names | Acetyl-CoA carboxylase 1, ACC1, ACC-alpha, Biotin carboxylase, ACACA, ACAC, ACC1, ACCA |
| Target/Specificity | This ACACA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2010-2039 amino acids from the C-terminal region of human ACACA. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ACACA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ACACA (HGNC:84) |
|---|---|
| Synonyms | ACAC, ACC1, ACCA |
| Function | Cytosolic enzyme that catalyzes the carboxylation of acetyl- CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis (PubMed:20457939, PubMed:20952656, PubMed:29899443). This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA (PubMed:20457939, PubMed:20952656, PubMed:29899443). |
| Cellular Location | Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q5SWU9} |
| Tissue Location | Expressed in brain, placenta, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Kim, C.W., et al. Proc. Natl. Acad. Sci. U.S.A. 107(21):9626-9631(2010)
Zhao, L.F., et al. Endocr. J. 57(4):317-324(2010)
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