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CLCN3 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P51790 |
| Other Accession | P51792, O18894, P51791, NP_776297.2, NP_001820.2 |
| Reactivity | Human |
| Predicted | Mouse, Rabbit, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 90966 Da |
| Antigen Region | 285-313 aa |
| Gene ID | 1182 |
|---|---|
| Other Names | H(+)/Cl(-) exchange transporter 3, Chloride channel protein 3, ClC-3, Chloride transporter ClC-3, CLCN3 |
| Target/Specificity | This CLCN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285-313 amino acids from the Central region of human CLCN3. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CLCN3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CLCN3 |
|---|---|
| Function | [Isoform 1]: Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons (By similarity). The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons (PubMed:29845874). The presence of conserved gating glutamate residues is typical for family members that function as antiporters (PubMed:29845874). |
| Cellular Location | [Isoform 1]: Early endosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Lysosome membrane {ECO:0000250|UniProtKB:P51791}; Multi-pass membrane protein. Cell membrane {ECO:0000250|UniProtKB:P51792}; Multi-pass membrane protein. Note=Isoform 1 is localized mainly in late endosomes. |
| Tissue Location | Expressed primarily in tissues derived from neuroectoderm. Within the brain, its expression is particularly evident in the hippocampus, olfactory cortex, and olfactory bulb. Highly expressed in aortic and coronary vascular smooth muscle cells, and aortic endothelial cells. Also expressed in tracheal and alveolar epithelial cells, and intima and media of the pulmonary vessels Expressed in bronchus and colon (at protein level) |
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Provided below are standard protocols that you may find useful for product applications.
Background
CLCN3 mediates the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could help neuronal cells to establish short-term memory.
References
Cuddapah, V.A., et al. J. Biol. Chem. 285(15):11188-11196(2010)
Xiong, D., et al. Clin. Exp. Pharmacol. Physiol. 36(4):386-393(2009)
Yin, Z., et al. Am. J. Physiol., Cell Physiol. 294 (2), C535-C542 (2008) :
Matsuda, J.J., et al. Am. J. Physiol., Cell Physiol. 294 (1), C251-C262 (2008) :
Kasinathan, R.S., et al. FEBS Lett. 581(28):5407-5412(2007)
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