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ORAI3 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9BRQ5 |
| Other Accession | NP_689501.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 31499 Da |
| Antigen Region | 1-30 aa |
| Gene ID | 93129 |
|---|---|
| Other Names | Protein orai-3, Transmembrane protein 142C, ORAI3, TMEM142C |
| Target/Specificity | This ORAI3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ORAI3. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ORAI3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ORAI3 |
|---|---|
| Synonyms | TMEM142C |
| Function | Pore-forming subunit of two major inward rectifying Ca(2+) channels at the plasma membrane: Ca(2+) release-activated Ca(2+) (CRAC) channels and arachidonate-regulated Ca(2+)-selective (ARC) channels (PubMed:16807233, PubMed:17442569, PubMed:19182790, PubMed:19622606, PubMed:19706554, PubMed:20354224, PubMed:32415068). Assembles with ORAI1 and ORAI2 to form hexameric CRAC channels that mediate Ca(2+) influx upon depletion of endoplasmic reticulum Ca(2+) store and channel activation by Ca(2+) sensor STIM1, a process known as store-operated Ca(2+) entry (SOCE). Various pore subunit combinations may account for distinct CRAC channel spatiotemporal and cell-type specific dynamics. ORAI1 mainly contributes to the generation of Ca(2+) plateaus involved in sustained Ca(2+) entry and is dispensable for cytosolic Ca(2+) oscillations, whereas ORAI2 and ORAI3 generate oscillatory patterns. CRAC channels assemble in Ca(2+) signaling microdomains where Ca(2+) influx is coupled to calmodulin and calcineurin signaling and activation of NFAT transcription factors recruited to ORAI1 via AKAP5. CRAC channels are the main pathway for Ca(2+) influx in T cells and promote the immune response to pathogens by activating NFAT-dependent cytokine and chemokine transcription (PubMed:16807233, PubMed:17442569, PubMed:19182790, PubMed:19706554, PubMed:20354224, PubMed:32415068). Assembles with ORAI1 to form channels that mediate store-independent Ca(2+) influx in response to inflammatory metabolites arachidonate or its derivative leukotriene C4, termed ARC and LRC channels respectively (PubMed:19622606, PubMed:32415068). |
| Cellular Location | Cell membrane; Multi-pass membrane protein. Note=Colocalizes with STIM1 upon store depletion. |
| Tissue Location | Expressed in both naive and effector T helper cells with higher levels in effector cells. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Key regulator or component of store-operated Ca(2+) channel and transcription factor NFAT nuclear import (By similarity).
References
Motiani, R.K., et al. J. Biol. Chem. 285(25):19173-19183(2010)
Bogeski, I., et al. Sci Signal 3 (115), RA24 (2010) :
Schindl, R., et al. Proc. Natl. Acad. Sci. U.S.A. 106(46):19623-19628(2009)
Lee, K.P., et al. Proc. Natl. Acad. Sci. U.S.A. 106(34):14687-14692(2009)
Frischauf, I., et al. J. Biol. Chem. 284(32):21696-21706(2009)
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