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ZP3 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P21754 |
| Other Accession | NP_001103824.1, NP_009086.4 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 47018 Da |
| Antigen Region | 353-380 aa |
| Gene ID | 7784 |
|---|---|
| Other Names | Zona pellucida sperm-binding protein 3, Sperm receptor, ZP3A/ZP3B, Zona pellucida glycoprotein 3, Zp-3, Zona pellucida protein C, Processed zona pellucida sperm-binding protein 3, ZP3, ZP3A, ZP3B, ZPC |
| Target/Specificity | This ZP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 353-380 amino acids from the C-terminal region of human ZP3. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ZP3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ZP3 |
|---|---|
| Synonyms | ZP3A, ZP3B, ZPC |
| Function | Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation. |
| Cellular Location | [Processed zona pellucida sperm-binding protein 3]: Zona pellucida |
| Tissue Location | Expressed in oocytes (at protein level). |
Provided below are standard protocols that you may find useful for product applications.
Background
The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene.
References
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Bansal, P., et al. Biol. Reprod. 81(1):7-15(2009)
Choudhury, S., et al. J. Reprod. Immunol. 79(2):137-147(2009)
Chiu, P.C., et al. Biol. Reprod. 79(5):869-877(2008)
Tormala, R.M., et al. Mol. Cell. Endocrinol. 289 (1-2), 10-15 (2008) :
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