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NARF Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9UHQ1 |
| Other Accession | NP_001033707.1, NP_001077077.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 51156 Da |
| Antigen Region | 134-160 aa |
| Gene ID | 26502 |
|---|---|
| Other Names | Nuclear prelamin A recognition factor, Iron-only hydrogenase-like protein 2, IOP2, NARF |
| Target/Specificity | This NARF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-160 amino acids from the Central region of human NARF. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NARF Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NARF |
|---|---|
| Cellular Location | Nucleus. |
| Tissue Location | Ubiquitous. Predominantly expressed in skeletal muscle, heart and brain. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Moller-Krull, M., et al. J. Mol. Biol. 382(3):601-609(2008)
Melzer, D., et al. PLoS Genet. 4 (5), E1000072 (2008) :
Lattanzi, G., et al. J. Cell. Biochem. 102(5):1149-1159(2007)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
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