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IER5 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 3
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q5VY09 |
| Other Accession | NP_057629.2 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 33704 Da |
| Antigen Region | 122-149 aa |
| Gene ID | 51278 |
|---|---|
| Other Names | Immediate early response gene 5 protein, IER5 |
| Target/Specificity | This IER5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-149 amino acids from the Central region of human IER5. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | IER5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | IER5 |
|---|---|
| Function | Plays a role as a transcription factor (PubMed:22132193, PubMed:25355627). Mediates positive transcriptional regulation of several chaperone genes during the heat shock response in a HSF1- dependent manner (PubMed:25355627, PubMed:25816751). Mediates negative transcriptional regulation of CDC25B expression (PubMed:22132193). Plays a role in the dephosphorylation of the heat shock factor HSF1 and ribosomal protein S6 kinase (S6K) by the protein phosphatase PP2A (PubMed:25816751, PubMed:26496226). Involved in the regulation of cell proliferation and resistance to thermal stress (PubMed:22132193, PubMed:25355627, PubMed:26496226). Involved in the cell cycle checkpoint and survival in response to ionizing radiation (PubMed:19238419, PubMed:22132193). Associates with chromatin to the CDC25B promoter (PubMed:22132193). |
| Cellular Location | Nucleus. Cytoplasm. Note=Predominantly cytoplasmic (PubMed:25816751). Translocated in the nucleus during heat shock (PubMed:25816751). |
| Tissue Location | Expressed in acute myeloid leukemia (AML) cells. |
Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a protein that is similar to other immediate early response proteins. In the mouse, a similar gene may play an important role in mediating the cellular response to mitogenic signals. Studies in rats found the expression of a similar gene to be increased after waking and sleep deprivation.
References
Ding, K.K., et al. Radiat Environ Biophys 48(2):205-213(2009)
Wan, D., et al. Proc. Natl. Acad. Sci. U.S.A. 101(44):15724-15729(2004)
Cirelli, C., et al. Brain Res. 885(2):303-321(2000)
Williams, M., et al. Genomics 55(3):327-334(1999)
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