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GPR84 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9NQS5 |
| Other Accession | NP_065103.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 43705 Da |
| Antigen Region | 265-293 aa |
| Gene ID | 53831 |
|---|---|
| Other Names | G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33, GPR84, EX33 |
| Target/Specificity | This GPR84 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-293 amino acids from the Central region of human GPR84. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | GPR84 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GPR84 |
|---|---|
| Synonyms | EX33 |
| Function | G protein-coupled receptor that responds endogenously to dietary fatty acids or nutrient, specifically medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists (PubMed:16966319). In immune cells, functions as a pro- inflammatory receptor via 6-OAU and promotes the expression of pro- inflammatory mediators such as TNFalpha, IL-6 and IL-12B as well as stimulating chemotactic responses through activation of signaling mediators AKT, ERK and NF-kappa-B (By similarity). In addition, triggers increased bacterial adhesion and phagocytosis in macrophages and regulates pro-inflammatory function via enhancing NLRP3 inflammasome activation (By similarity). Plays also an important role in inflammation by modulating neutrophil functions (By similarity). Mechanistically, promotes neutrophil chemotaxis, reactive oxygen species (ROS) production and degranulation via LYN-AKT/ERK pathway (By similarity). To regulate ROS, communicates with the two formyl peptide receptors FPR2 and FPR1 to control the NADPH oxidase activity in neutrophils (PubMed:33789297). |
| Cellular Location | Cell membrane; Multi-pass membrane protein |
| Tissue Location | Expressed predominantly in hematopoietic tissues. High levels detected in the bone marrow and lower levels in the peripheral leukocytes and lung. Also expressed in brain, heart, muscle, colon, thymus, splen, kidney, liver, placenta and intestine. Within the leukocyte population expression is higher in neutrophils and eosinophils relative to T- or B-lymphocytes |
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Provided below are standard protocols that you may find useful for product applications.
Background
Receptor for medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists. Not activated by short-chain and long-chain saturated and unsaturated FFAs. Activation by medium-chain free fatty acid is coupled to a pertussis toxin sensitive G(i/o) protein pathway. May have important roles in processes from fatty acid metabolism to regulation of the immune system.
References
Takeda, S., et al. FEBS Lett. 520 (1-3), 97-101 (2002) :
Yousefi, S., et al. J. Leukoc. Biol. 69(6):1045-1052(2001)
Wittenberger, T., et al. J. Mol. Biol. 307(3):799-813(2001)
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