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PAFAH2 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q99487 |
| Other Accession | Q8VDG7, NP_000428.2 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 44036 Da |
| Antigen Region | 249-276 aa |
| Gene ID | 5051 |
|---|---|
| Other Names | Platelet-activating factor acetylhydrolase 2, cytoplasmic, Serine-dependent phospholipase A2, SD-PLA2, hSD-PLA2, PAFAH2 |
| Target/Specificity | This PAFAH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 249-276 amino acids from the Central region of human PAFAH2. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PAFAH2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PAFAH2 (HGNC:8579) |
|---|---|
| Function | Catalyzes the hydrolyze of the acetyl group at the sn-2 position of platelet-activating factor (PAF) and its analogs, leading to their inactivation (PubMed:9494101). Hydrolyzes propionyl and butyroyl moieties approximately half as effectively as PAF (By similarity). Also catalyzes transacetylation of the acetyl group from platelet-activating factor (PAF) to lysoplasmalogen and to sphingosine, producing plasmalogen analogs of PAF and N-acetylsphingosine (C2- ceramide) respectively. Has a marked selectivity for phospholipids with short acyl chains at the sn-2 position (By similarity). |
| Cellular Location | Cytoplasm. Membrane {ECO:0000250|UniProtKB:P79106}; Lipid-anchor {ECO:0000250|UniProtKB:P79106}. Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:P79106}; Lipid-anchor {ECO:0000250|UniProtKB:P79106}. Note=In resting cells, localizes to intracellular membranes and cytoplasm. Translocates from the cytoplasm to intracellular membranes upon oxidative stress {ECO:0000250|UniProtKB:P79106} |
| Tissue Location | Broadly expressed in different tissues, but high in B- and T-lymphocytes. In brain, expression is restricted to amygdala and frontal cortex. |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes platelet-activating factor acetylhydrolase isoform 2, a single-subunit intracellular enzyme that catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). However, this lipase exhibits a broader substrate specificity than simply platelet activating factor. Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist, and both are multi-subunit enzymes. Additionally, there is a single-subunit serum isoform of this enzyme.
References
Schmidt, E.B., et al. Atherosclerosis 196(1):420-424(2008)
Umemura, K., et al. Stroke 38(3):1063-1068(2007)
Unno, N., et al. J. Surg. Res. 134(1):36-43(2006)
Marques, M., et al. J. Invest. Dermatol. 119(4):913-919(2002)
Min, J.H., et al. Biochemistry 40(15):4539-4549(2001)
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