PPP1R16B Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 3
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
---|---|
Primary Accession | Q96T49 |
Other Accession | Q8VHQ3, Q95N27, NP_001166206.1 |
Reactivity | Human |
Predicted | Bovine, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 63551 Da |
Antigen Region | 372-399 aa |
Gene ID | 26051 |
---|---|
Other Names | Protein phosphatase 1 regulatory inhibitor subunit 16B, Ankyrin repeat domain-containing protein 4, CAAX box protein TIMAP, TGF-beta-inhibited membrane-associated protein, hTIMAP, PPP1R16B, ANKRD4, KIAA0823 |
Target/Specificity | This PPP1R16B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 372-399 amino acids from the Central region of human PPP1R16B. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | PPP1R16B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PPP1R16B |
---|---|
Synonyms | ANKRD4, KIAA0823 |
Function | Regulator of protein phosphatase 1 (PP1) that acts as a positive regulator of pulmonary endothelial cell (EC) barrier function (PubMed:18586956). Involved in the regulation of the PI3K/AKT signaling pathway, angiogenesis and endothelial cell proliferation (PubMed:25007873). Regulates angiogenesis and endothelial cell proliferation through the control of ECE1 dephosphorylation, trafficking and activity (By similarity). Protects the endothelial barrier from lipopolysaccharide (LPS)-induced vascular leakage (By similarity). Involved in the regulation of endothelial cell filopodia extension (By similarity). May be a downstream target for TGF-beta1 signaling cascade in endothelial cells (PubMed:16263087, PubMed:18586956). Involved in PKA-mediated moesin dephosphorylation which is important in EC barrier protection against thrombin stimulation (PubMed:18586956). Promotes the interaction of PPP1CA with RPSA/LAMR1 and in turn facilitates the dephosphorylation of RPSA/LAMR1 (PubMed:16263087). Involved in the dephosphorylation of EEF1A1 (PubMed:26497934). |
Cellular Location | Cell membrane. Cell membrane; Lipid-anchor. Nucleus. Cell projection. Note=Colocalizes with RPSA/LAMR1 in the cell membrane (PubMed:16263087). Localizes to the perinuclear region (By similarity). Colocalizes with PTEN at the tip of EC projections (PubMed:25007873). {ECO:0000250|UniProtKB:Q95N27, ECO:0000269|PubMed:16263087, ECO:0000269|PubMed:25007873} |
Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is membrane-associated and contains five ankyrin repeats, a protein phosphatase-1-interacting domain, and a carboxy-terminal CAAX box domain. Synthesis of the encoded protein is inhibited by transforming growth factor beta-1. The protein may bind to the membrane through its CAAX box domain and may act as a signaling molecule through interaction with protein phosphatase-1. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.
References
Csortos, C., et al. Am. J. Physiol. Lung Cell Mol. Physiol. 295 (3), L440-L450 (2008) :
Kim, K., et al. Biochem. Biophys. Res. Commun. 338(3):1327-1334(2005)
Homma, K., et al. J. Mol. Biol. 343(5):1207-1220(2004)
Cao, W., et al. Am. J. Physiol., Cell Physiol. 283 (1), C327-C337 (2002) :
Deloukas, P., et al. Nature 414(6866):865-871(2001)
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.