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NHEDC2 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q86UD5 |
| Other Accession | Q5BKR2, NP_849155.2 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 57564 Da |
| Antigen Region | 458-486 aa |
| Gene ID | 133308 |
|---|---|
| Other Names | Mitochondrial sodium/hydrogen exchanger 9B2, Mitochondrial Na(+)/H(+) exchanger NHA2, Na(+)/H(+) exchanger-like domain-containing protein 2, NHE domain-containing protein 2, Sodium/hydrogen exchanger-like domain-containing protein 2, Solute carrier family 9 subfamily B member 2, SLC9B2, NHA2, NHEDC2 |
| Target/Specificity | This NHEDC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 458-486 amino acids from the C-terminal region of human NHEDC2. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NHEDC2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SLC9B2 (HGNC:25143) |
|---|---|
| Synonyms | NHA2, NHEDC2 |
| Function | Electroneutral Na(+) Li(+)/H(+) antiporter that extrudes Na(+) or Li(+) in exchange for external protons across the membrane (PubMed:18000046, PubMed:18508966, PubMed:22948142, PubMed:28154142, PubMed:36177733). Uses the proton gradient/membrane potential to extrude sodium (PubMed:22948142). Contributes to the regulation of intracellular pH and sodium homeostasis (By similarity). Also able to mediate Na(+)/Li(+) antiporter activity in kidney (PubMed:22948142). May play a physiological role in renal tubular function and blood pressure homeostasis (By similarity). Plays an important role for insulin secretion and clathrin-mediated endocytosis in beta-cells (By similarity). Involved in sperm motility and fertility (By similarity). It is controversial whether SLC9B2 plays a role in osteoclast differentiation or not (By similarity). |
| Cellular Location | Cell membrane; Multi-pass membrane protein {ECO:0000250|UniProtKB:A0A6P3HVI0}. Mitochondrion membrane {ECO:0000250|UniProtKB:Q5BKR2}; Multi-pass membrane protein {ECO:0000250|UniProtKB:A0A6P3HVI0}. Endosome membrane {ECO:0000250|UniProtKB:Q5BKR2}; Multi-pass membrane protein {ECO:0000250|UniProtKB:A0A6P3HVI0}. Recycling endosome membrane {ECO:0000250|UniProtKB:Q5BKR2}; Multi-pass membrane protein {ECO:0000250|UniProtKB:A0A6P3HVI0}. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000250|UniProtKB:Q5BKR2}; Multi-pass membrane protein {ECO:0000250|UniProtKB:A0A6P3HVI0}. Cell projection, cilium, flagellum membrane {ECO:0000250|UniProtKB:Q5BKR2}; Multi-pass membrane protein {ECO:0000250|UniProtKB:A0A6P3HVI0} Basolateral cell membrane {ECO:0000250|UniProtKB:Q5BKR2}; Multi-pass membrane protein {ECO:0000250|UniProtKB:A0A6P3HVI0}. Apical cell membrane {ECO:0000250|UniProtKB:Q5BKR2}; Multi-pass membrane protein {ECO:0000250|UniProtKB:A0A6P3HVI0}. Note=Strong colocalization with LAMP1 and TCIRG1 in osteoclasts. In beta-cells colocalizes with RAB4A and SYP. Localizes to the basolateral membrane of polarized osteoclasts. {ECO:0000250|UniProtKB:Q5BKR2} |
| Tissue Location | Widely expressed (PubMed:18508966). High levels detected in the distal tubules of the kidney nephron (PubMed:18508966) Detected in red blood cells (at protein level) (PubMed:18000046, PubMed:18508966). |
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Provided below are standard protocols that you may find useful for product applications.
Background
Sodium hydrogen antiporters, such as NHEDC2, convert the proton motive force established by the respiratory chain or the F1F0 mitochondrial ATPase into sodium gradients that drive other energy-requiring processes, transduce environmental signals into cell responses, or function in drug efflux (Xiang et al., 2007 [PubMed 18000046]).
References
Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010)
Schushan, M., et al. J. Mol. Biol. 396(5):1181-1196(2010)
Fuster, D.G., et al. J. Am. Soc. Nephrol. 19(8):1547-1556(2008)
Battaglino, R.A., et al. Bone 42(1):180-192(2008)
Xiang, M., et al. Proc. Natl. Acad. Sci. U.S.A. 104(47):18677-18681(2007)
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