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NTAN1 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q96AB6 |
| Other Accession | NP_775745.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 34677 Da |
| Antigen Region | 203-230 aa |
| Gene ID | 123803 |
|---|---|
| Other Names | Protein N-terminal asparagine amidohydrolase, 351-, Protein NH2-terminal asparagine amidohydrolase, PNAA, Protein NH2-terminal asparagine deamidase, PNAD, Protein N-terminal Asn amidase, Protein N-terminal asparagine amidase, Protein NTN-amidase, NTAN1 |
| Target/Specificity | This NTAN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 203-230 amino acids from the C-terminal region of human NTAN1. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NTAN1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NTAN1 |
|---|---|
| Function | N-terminal asparagine deamidase that mediates deamidation of N-terminal asparagine residues to aspartate. Required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. These proteins are acetylated on the retained initiator methionine and can subsequently be modified by the removal of N-acetyl methionine by acylaminoacid hydrolase (AAH). Conversion of the resulting N-terminal asparagine to aspartate by NTAN1/PNAD renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position, nor on acetylated N-terminal peptidyl Asn. |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:Q28955}. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Side-chain deamidation of N-terminal asparagine residues to aspartate. Required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. These proteins are acetylated on the retained initiator methionine and can subsequently be modified by the removal of N-acetyl methionine by acylaminoacid hydrolase (AAH). Conversion of the resulting N-terminal asparagine to aspartate by PNAD renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position (By similarity).
References
Okada, Y., et al. Hum. Mol. Genet. 19(11):2303-2312(2010)
Kamdem, L.K., et al. Pharmacogenet. Genomics 18(6):507-514(2008)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Grigoryev, S., et al. J. Biol. Chem. 271(45):28521-28532(1996)
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