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ERN1 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | O75460 |
| Other Accession | NP_001424.3 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 109735 Da |
| Antigen Region | 828-857 aa |
| Gene ID | 2081 |
|---|---|
| Other Names | Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1, Inositol-requiring protein 1, hIRE1p, Ire1-alpha, IRE1a, Serine/threonine-protein kinase, Endoribonuclease, 3126-, ERN1 (HGNC:3449) |
| Target/Specificity | This ERN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 828-857 amino acids from the C-terminal region of human ERN1. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ERN1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ERN1 (HGNC:3449) |
|---|---|
| Function | Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR) (PubMed:11175748, PubMed:11779464, PubMed:12637535, PubMed:21317875, PubMed:28128204, PubMed:30118681, PubMed:9637683, PubMed:19328063). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP (PubMed:21317875). Accumulation of misfolded proteins in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity (PubMed:21317875). The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA (PubMed:11779464, PubMed:21317875, PubMed:24508390). The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes (PubMed:11779464, PubMed:21317875, PubMed:24508390). Acts as an upstream signal for ER stress-induced GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane by modulating the expression and localization of SEC16A (PubMed:21884936, PubMed:28067262). |
| Cellular Location | Endoplasmic reticulum membrane; Single-pass type I membrane protein |
| Tissue Location | Ubiquitously expressed. High levels observed in pancreatic tissue. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals.
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Li, H., et al. Proc. Natl. Acad. Sci. U.S.A. 107(37):16113-16118(2010)
Auf, G., et al. Proc. Natl. Acad. Sci. U.S.A. 107(35):15553-15558(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Gupta, S., et al. PLoS Biol. 8 (7), E1000410 (2010) :
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