ARSA Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | O43681 |
Other Accession | O54984, A5PJI5, NP_004308.2, G3V9T7 |
Reactivity | Human |
Predicted | Bovine, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 38793 Da |
Antigen Region | 284-312 aa |
Gene ID | 439 |
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Other Names | ATPase ASNA1 {ECO:0000255|HAMAP-Rule:MF_03112}, 36-- {ECO:0000255|HAMAP-Rule:MF_03112}, Arsenical pump-driving ATPase {ECO:0000255|HAMAP-Rule:MF_03112}, Arsenite-stimulated ATPase {ECO:0000255|HAMAP-Rule:MF_03112}, Transmembrane domain recognition complex 40 kDa ATPase subunit, hARSA-I, hASNA-I, ASNA1 {ECO:0000255|HAMAP-Rule:MF_03112}, ARSA, TRC40 |
Target/Specificity | This ARSA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 284-312 amino acids from the C-terminal region of human ARSA. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | ARSA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | GET3 {ECO:0000255|HAMAP-Rule:MF_03112, ECO:0000312|HGNC:HGNC:752} |
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Function | ATPase required for the post-translational delivery of tail- anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1/WRB and CAMLG/GET2, where the tail- anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-CAMLG receptor, and returning it to the cytosol to initiate a new round of targeting. May be involved in insulin signaling. |
Cellular Location | Cytoplasm. Endoplasmic reticulum. Nucleus, nucleolus |
Tissue Location | Expressed in the epithelial cells of the liver, kidney, and stomach wall, in the adrenal medulla, in the islet cells of the pancreas, in the red pulp of the spleen, and in cardiac and skeletal muscle. |
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Provided below are standard protocols that you may find useful for product applications.
Background
ASNA1 is the human homolog of the bacterial arsA gene. In E. coli, ArsA ATPase is the catalytic component of a multisubunit oxyanion pump that is responsible for resistance to arsenicals and antimonials.
References
Favaloro, V., et al. J. Cell. Sci. 123 (PT 9), 1522-1530 (2010) :
Hemmingsson, O., et al. Oncol. Rep. 22(4):869-875(2009)
Rabu, C., et al. J. Biol. Chem. 283(41):27504-27513(2008)
Stefanovic, S., et al. Cell 128(6):1147-1159(2007)
Kao, G., et al. Cell 128(3):577-587(2007)
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