DUS2L Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q9NX74 |
Other Accession | NP_060273.1 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 55050 Da |
Antigen Region | 371-397 aa |
Gene ID | 54920 |
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Other Names | tRNA-dihydrouridine(20) synthase [NAD(P)+]-like, 131-, Dihydrouridine synthase 2, Up-regulated in lung cancer protein 8, URLC8, tRNA-dihydrouridine synthase 2-like, hDUS2, DUS2, DUS2L |
Target/Specificity | This DUS2L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 371-397 amino acids from the C-terminal region of human DUS2L. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | DUS2L Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | DUS2 |
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Synonyms | DUS2L |
Function | Dihydrouridine synthase. Catalyzes the NADPH-dependent synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs (PubMed:15994936, PubMed:26429968, PubMed:30149704, PubMed:34798057). Negatively regulates the activation of EIF2AK2/PKR (PubMed:18096616). |
Cellular Location | Cytoplasm. Endoplasmic reticulum. Note=Mainly at the endoplasmic reticulum. |
Tissue Location | Weak expression in heart, placenta and skeletal muscle. Up-regulated in most lung cancer cells (at protein level) |
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Provided below are standard protocols that you may find useful for product applications.
Background
Dihydrouridine synthase catalyzes reduction of the 5,6-double bond of a uridine residue on the displacement loop of tRNA. The resultant modified base, 5,6-dihydrouridine, appears to increase the conformational flexibility and dynamic motion of tRNA (Kato et al., 2005 [PubMed 15994936]).
References
Mittelstadt, M., et al. Nucleic Acids Res. 36(3):998-1008(2008)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Kato, T., et al. Cancer Res. 65(13):5638-5646(2005)
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