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> home > Products > Primary Antibodies > Antibody Collections > Ubiquitin E3 Ligases (RING Domain) Antibodies > RNF145 Antibody (C-term)
RNF145 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q96MT1 |
| Other Accession | NP_653327.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 75617 Da |
| Antigen Region | 601-627 aa |
| Gene ID | 153830 |
|---|---|
| Other Names | RING finger protein 145, RNF145 |
| Target/Specificity | This RNF145 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 601-627 amino acids of human RNF145. |
| Dilution | WB~~1:2000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | RNF145 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RNF145 {ECO:0000312|PROSITE:PS50089} |
|---|---|
| Function | E3 ubiquitin ligase that catalyzes the direct transfer of ubiquitin from E2 ubiquitin-conjugating enzyme to a specific substrate. In response to bacterial infection, negatively regulates the phagocyte oxidative burst by controlling the turnover of the NADPH oxidase complex subunits. Promotes monoubiquitination of CYBA and 'Lys-48'- linked polyubiquitination and degradation of CYBB NADPH oxidase catalytic subunits, both essential for the generation of antimicrobial reactive oxygen species. Involved in the maintenance of cholesterol homeostasis. In response to high sterol concentrations ubiquitinates HMGCR, a rate-limiting enzyme in cholesterol biosynthesis, and targets it for degradation. The interaction with INSIG1 is required for this function. In addition, triggers ubiquitination of SCAP, likely inhibiting its transport to the Golgi apparatus and the subsequent processing/maturation of SREBPF2, ultimately down-regulating cholesterol biosynthesis. |
| Cellular Location | Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q5SWK7}; Multi-pass membrane protein |
Research Areas
Citations ( 0 )
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
The function of this protein remains unknown.
References
Soranzo, N., et al. Nat. Genet. 41(11):1182-1190(2009)
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$ 150.00
$ 385.00
Cat# AP18281B
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