ALKBH5 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, FC, IHC-P, E |
---|---|
Primary Accession | Q6P6C2 |
Other Accession | D3ZKD3, Q3TSG4, E1BH29, NP_060228.3 |
Reactivity | Human |
Predicted | Bovine, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 44256 Da |
Antigen Region | 302-330 aa |
Gene ID | 54890 |
---|---|
Other Names | RNA demethylase ALKBH5, 11411-, Alkylated DNA repair protein alkB homolog 5, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5, ALKBH5, ABH5, OFOXD1 |
Target/Specificity | This ALKBH5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 302-330 amino acids from the Central region of human ALKBH5. |
Dilution | WB~~1:2000 IHC-P~~1:100 FC~~1:25 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | ALKBH5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ALKBH5 |
---|---|
Synonyms | ABH5, OFOXD1 |
Function | Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:23177736, PubMed:24489119, PubMed:24616105, PubMed:24778178). Can also demethylate N(6)-methyladenosine in single- stranded DNA (in vitro) (PubMed:24616105). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:21264265, PubMed:23177736, PubMed:24489119, PubMed:24616105, PubMed:24778178). Demethylation of m6A mRNA affects mRNA processing and export (PubMed:23177736). Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3'-UTR mRNAs in male germ cells (By similarity). |
Cellular Location | Nucleus speckle |
Tissue Location | Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary |
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Provided below are standard protocols that you may find useful for product applications.
Background
Probable dioxygenase that requires molecular oxygen, alpha-ketoglutarate and iron (By similarity).
References
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Bi, W., et al. Genome Res. 12(5):713-728(2002)
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