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GCLC Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P48506 |
| Other Accession | P19468, P97494, NP_001489.1 |
| Reactivity | Human, Mouse |
| Predicted | Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 72766 Da |
| Antigen Region | 286-315 aa |
| Gene ID | 2729 |
|---|---|
| Other Names | Glutamate--cysteine ligase catalytic subunit, GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase, GCLC, GLCL, GLCLC |
| Target/Specificity | This GCLC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the Central region of human GCLC. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | GCLC Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GCLC (HGNC:4311) |
|---|---|
| Synonyms | GLCL, GLCLC |
| Function | Catalyzes the ATP-dependent ligation of L-glutamate and L- cysteine and participates in the first and rate-limiting step in glutathione biosynthesis. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
References
Man, B.L., et al. J Clin Neurosci 17(10):1244-1247(2010)
Jia, P., et al. Schizophr. Res. 122 (1-3), 38-42 (2010) :
Le, T.M., et al. Mol. Genet. Metab. 101(1):55-61(2010)
Fullerton, J.M., et al. Bipolar Disord 12(5):550-556(2010)
Wang, X., et al. PLoS ONE 5 (8), E11934 (2010) :
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