ALG8 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q9BVK2 |
Other Accession | Q6P8H8, Q0P5D9, NP_076984.2 |
Reactivity | Human |
Predicted | Bovine, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 60088 Da |
Antigen Region | 15-41 aa |
Gene ID | 79053 |
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Other Names | Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1, 3-glucosyltransferase, Asparagine-linked glycosylation protein 8 homolog, Dol-P-Glc:Glc(1)Man(9)GlcNAc(2)-PP-dolichyl alpha-1, 3-glucosyltransferase, Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase, ALG8 |
Target/Specificity | This ALG8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-41 amino acids from the N-terminal region of human ALG8. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | ALG8 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ALG8 {ECO:0000303|PubMed:28375157, ECO:0000312|HGNC:HGNC:23161} |
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Function | Adds the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc(1)Man(9)GlcNAc(2)-PP-Dol before it is transferred to the nascent peptide (By similarity). Required for PKD1/Polycystin-1 maturation and localization to the plasma membrane of the primary cilia (By similarity). |
Cellular Location | Endoplasmic reticulum membrane; Multi-pass membrane protein |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a member of the ALG6/ALG8 glucosyltransferase family. The encoded protein catalyzes the addition of the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation of proteins. Mutations in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ih). Alternatively spliced transcript variants encoding different isoforms have been identified.
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Stolting, T., et al. Mol. Genet. Metab. 98(3):305-309(2009)
Jaeken, J., et al. Curr. Opin. Pediatr. 16(4):434-439(2004)
Schollen, E., et al. J. Med. Genet. 41(7):550-556(2004)
Jaeken, J. J. Inherit. Metab. Dis. 27(3):423-426(2004)
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