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MKLN1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9UL63 |
| Other Accession | Q99PV3, O89050, NP_001138826.1 |
| Reactivity | Human |
| Predicted | Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 84768 Da |
| Antigen Region | 300-326 aa |
| Gene ID | 4289 |
|---|---|
| Other Names | Muskelin, MKLN1 |
| Target/Specificity | This MKLN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300-326 amino acids from the Central region of human MKLN1. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | MKLN1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MKLN1 |
|---|---|
| Function | Component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1 (PubMed:29911972). Required for internalization of the GABA receptor GABRA1 from the cell membrane via endosomes and subsequent GABRA1 degradation (By similarity). Acts as a mediator of cell spreading and cytoskeletal responses to the extracellular matrix component THBS1 (PubMed:18710924). |
| Cellular Location | Cytoplasm. Cytoplasm, cytosol {ECO:0000250|UniProtKB:O89050}. Nucleus, nucleoplasm {ECO:0000250|UniProtKB:O89050}. Cell projection, ruffle {ECO:0000250|UniProtKB:O89050}. Cytoplasm, cell cortex {ECO:0000250|UniProtKB:O89050}. Synapse {ECO:0000250|UniProtKB:O89050} Postsynapse {ECO:0000250|UniProtKB:O89050}. Note=Colocalizes with GABRA1 at synapses and in postsynaptic regions. Colocalizes with actin fibers in the cell cortex. {ECO:0000250|UniProtKB:O89050} |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Muskelin is an intracellular protein that acts as a mediator of cell spreading and cytoskeletal responses to the extracellular matrix component thrombospondin I (MIM 188060) (Adams et al., 1998 [PubMed 9724633]).
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Shyn, S.I., et al. Mol. Psychiatry (2009) In press :
Valiyaveettil, M., et al. J. Cell Biol. 182(4):727-739(2008)
Kobayashi, N., et al. Gene 396(2):236-247(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
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