PELI1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
---|---|
Primary Accession | Q96FA3 |
Other Accession | Q8C669, NP_065702.2 |
Reactivity | Human |
Predicted | Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 46286 Da |
Antigen Region | 177-204 aa |
Gene ID | 57162 |
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Other Names | E3 ubiquitin-protein ligase pellino homolog 1, Pellino-1, 632-, Pellino-related intracellular-signaling molecule, PELI1, PRISM |
Target/Specificity | This PELI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-204 amino acids from the Central region of human PELI1. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | PELI1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PELI1 |
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Synonyms | PRISM |
Function | E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL- 1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation (PubMed:12496252, PubMed:17675297). Mediates 'Lys-48'-linked polyubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation; preferentially recognizes and mediates the degradation of the 'Thr-182' phosphorylated form of RIPK3 (PubMed:29883609). Negatively regulates necroptosis by reducing RIPK3 expression (PubMed:29883609). Mediates 'Lys-63'-linked ubiquitination of RIPK1 (PubMed:29883609). |
Tissue Location | Expressed at high levels in normal skin but decreased in keratinocytes from toxic epidermal necrolysis (TEN) patients (at protein level). |
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Provided below are standard protocols that you may find useful for product applications.
Background
Scaffold protein involved in the IL-1 signaling pathway via its interaction with the complex containing IRAK kinases and TRAF6. Required for NF-kappa-B activation and IL-8 gene expression in response to IL-1.
References
Ordureau, A., et al. Biochem. J. 409(1):43-52(2008)
Butler, M.P., et al. J. Biol. Chem. 282(41):29729-29737(2007)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Choi, K.C., et al. Nat. Immunol. 7(10):1057-1065(2006)
Butler, M.P., et al. J. Biol. Chem. 280(30):27759-27768(2005)
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