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Mouse Rnasen Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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- CITATIONS
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- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q5HZJ0 |
| Other Accession | NP_001123621.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 158828 Da |
| Antigen Region | 737-764 aa |
| Gene ID | 14000 |
|---|---|
| Other Names | Ribonuclease 3, Protein Drosha, Ribonuclease III, RNase III, Drosha, Etohi2, Rn3, Rnasen |
| Target/Specificity | This Mouse Rnasen antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 737-764 amino acids from the Central region of mouse Rnasen. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Mouse Rnasen Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Drosha |
|---|---|
| Synonyms | Etohi2, Rn3, Rnasen |
| Function | Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri- miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs (PubMed:26255770). Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies. |
| Cellular Location | Nucleus {ECO:0000250|UniProtKB:Q9NRR4}. Nucleus, nucleolus {ECO:0000250|UniProtKB:Q9NRR4}. Note=A fraction is translocated to the nucleolus during the S phase of the cell cycle Localized in GW bodies (GWBs), also known as P-bodies {ECO:0000250|UniProtKB:Q9NRR4} |
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Provided below are standard protocols that you may find useful for product applications.
Background
Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, RNASEN/DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies (By similarity).
References
Chong, M.M., et al. Genes Dev. 24(17):1951-1960(2010)
Yang, J.S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(34):15163-15168(2010)
Michon, F., et al. Dev. Biol. 340(2):355-368(2010)
Wu, H., et al. PLoS ONE 4 (10), E7566 (2009) :
Shenoy, A., et al. PLoS ONE 4 (9), E6971 (2009) :
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