ENAH Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
---|---|
Primary Accession | Q8N8S7 |
Other Accession | NP_060682.2 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 66510 Da |
Antigen Region | 446-475 aa |
Gene ID | 55740 |
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Other Names | Protein enabled homolog, ENAH, MENA |
Target/Specificity | This ENAH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 446-475 amino acids from the C-terminal region of human ENAH. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | ENAH Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ENAH |
---|---|
Synonyms | MENA |
Function | Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation (By similarity). |
Cellular Location | Cytoplasm. Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, filopodium. Synapse. Cell junction, focal adhesion. Note=Targeted to the leading edge of lamellipodia and filopodia by MRL family members. Colocalizes at filopodial tips with a number of other proteins including vinculin and zyxlin. Colocalizes with N-WASP at the leading edge. Colocalizes with GPHN and PFN at synapses (By similarity). |
Tissue Location | Expressed in myoepithelia of parotid, breast, bronchial glands and sweat glands. Expressed in colon-rectum muscolaris mucosae epithelium, pancreas acinar ductal epithelium, endometrium epithelium, prostate fibromuscolar stroma and placenta vascular media Overexpressed in a majority of breast cancer cell lines and primary breast tumor lesions. |
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Background
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. Required for the actin-based mobility of Listeria monocytogenes (By similarity).
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Chattopadhyay, I., et al. Mutat. Res. 696(2):130-138(2010)
Hahn, W.H., et al. Exp. Mol. Med. 41(11):793-801(2009)
Gurzu, S., et al. Rom J Morphol Embryol 50(2):213-216(2009)
Higashi, M., et al. PLoS ONE 4 (3), E4765 (2009) :
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