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MOCS3 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | O95396 |
| Other Accession | A2BDX3, NP_055299.1 |
| Reactivity | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 49669 Da |
| Antigen Region | 40-66 aa |
| Gene ID | 27304 |
|---|---|
| Other Names | Adenylyltransferase and sulfurtransferase MOCS3 {ECO:0000255|HAMAP-Rule:MF_03049}, Molybdenum cofactor synthesis protein 3 {ECO:0000255|HAMAP-Rule:MF_03049}, Molybdopterin synthase sulfurylase, MPT synthase sulfurylase, Molybdopterin-synthase adenylyltransferase {ECO:0000255|HAMAP-Rule:MF_03049}, 27780 {ECO:0000255|HAMAP-Rule:MF_03049}, Adenylyltransferase MOCS3 {ECO:0000255|HAMAP-Rule:MF_03049}, Sulfur carrier protein MOCS2A adenylyltransferase {ECO:0000255|HAMAP-Rule:MF_03049}, Molybdopterin-synthase sulfurtransferase {ECO:0000255|HAMAP-Rule:MF_03049}, 28111 {ECO:0000255|HAMAP-Rule:MF_03049}, Sulfur carrier protein MOCS2A sulfurtransferase {ECO:0000255|HAMAP-Rule:MF_03049}, Sulfurtransferase MOCS3 {ECO:0000255|HAMAP-Rule:MF_03049}, MOCS3 {ECO:0000255|HAMAP-Rule:MF_03049} |
| Target/Specificity | This MOCS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-66 amino acids from the N-terminal region of human MOCS3. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | MOCS3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MOCS3 {ECO:0000255|HAMAP-Rule:MF_03049} |
|---|---|
| Function | Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl- adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (- COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as a nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 acting as a sulfur donor for thiocarboxylation reactions. |
| Cellular Location | Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03049, ECO:0000269|PubMed:15073332, ECO:0000269|PubMed:23593335} |
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Provided below are standard protocols that you may find useful for product applications.
Background
Molybdenum cofactor (MoCo) is necessary for the function of all molybdoenzymes. One of the enzymes required for the biosynthesis of MoCo is molybdopterin synthase (MPT synthase). The protein encoded by this gene adenylates and activates MPT synthase. This gene contains no introns. A pseudogene of this gene is present on chromosome 14.
References
Schlieker, C.D., et al. Proc. Natl. Acad. Sci. U.S.A. 105(47):18255-18260(2008)
Marelja, Z., et al. J. Biol. Chem. 283(37):25178-25185(2008)
Schmitz, J., et al. Biochemistry 47(24):6479-6489(2008)
Krepinsky, K., et al. FEBS J. 274(11):2778-2787(2007)
Matthies, A., et al. Biochemistry 44(21):7912-7920(2005)
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