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HEPACAM2 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HEPACAM2 Antibody (C-term) AP19350b
    HEPACAM2 Antibody (C-term)(Cat. #AP19350b) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the HEPACAM2 antibody detected the HEPACAM2 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession A8MVW5
Other Accession NP_937794.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 51407 Da
Antigen Region 371-400 aa
Additional Information
Gene ID 253012
Other Names HEPACAM family member 2, Mitotic kinetics regulator, HEPACAM2, MIKI
Target/Specificity This HEPACAM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 371-400 amino acids from the C-terminal region of human HEPACAM2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHEPACAM2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HEPACAM2
Synonyms MIKI
Function Required during prometaphase for centrosome maturation. Following poly-ADP-ribosylation (PARsylation) by TNKS, translocates from the Golgi apparatus to mitotic centrosomes and plays a key role in the formation of robust microtubules for prompt movement of chromosomes: anchors AKAP9/CG-NAP, a scaffold protein of the gamma- tubulin ring complex and promotes centrosome maturation.
Cellular Location Golgi apparatus membrane; Single-pass type I membrane protein. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Midbody. Note=In interphase, localizes to the Golgi apparatus. Localizes to centrosomes and spindles during prophase, prometaphase, and metaphase of mitosis, and to midbodies at telophase Translocation to mitotic centrosomes is the result of poly-ADP- ribosylation (PARsylation).
Tissue Location Widely expressed..
Research Areas
Citations (0)
citation

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Background

The function of the HEPACAM2 protein remains unknown.

References

Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)

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$ 365.00
$ 140.00
Cat# AP19350b
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