RNF135 Antibody(C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q8IUD6 |
Other Accession | NP_115698.3 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 47888 Da |
Antigen Region | 375-401 aa |
Gene ID | 84282 |
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Other Names | E3 ubiquitin-protein ligase RNF135, 632-, RIG-I E3 ubiquitin ligase, REUL, RING finger protein 135, Riplet, RNF135 |
Target/Specificity | This RNF135 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 375-401 amino acids from the C-terminal region of human RNF135. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | RNF135 Antibody(C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | RNF135 (HGNC:21158) |
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Function | E2-dependent E3 ubiquitin-protein ligase that functions as a RIGI coreceptor in the sensing of viral RNAs in cell cytoplasm and the activation of the antiviral innate immune response (PubMed:19017631, PubMed:19484123, PubMed:21147464, PubMed:23950712, PubMed:28469175, PubMed:31006531). Together with the UBE2D3, UBE2N and UB2V1 E2 ligases, catalyzes the 'Lys-63'-linked polyubiquitination of RIGI oligomerized on viral RNAs, an essential step in the activation of the RIG-I signaling pathway (PubMed:19017631, PubMed:21147464, PubMed:28469175, PubMed:31006531). Through a ubiquitin-independent parallel mechanism, which consists in bridging RIGI filaments forming on longer viral RNAs, further activates the RIG-I signaling pathway (PubMed:31006531). This second mechanism that synergizes with the ubiquitin-dependent one would thereby allow an RNA length-dependent regulation of the RIG-I signaling pathway (Probable). Associated with the E2 ligase UBE2N, also constitutively synthesizes unanchored 'Lys-63'-linked polyubiquitin chains that may also activate the RIG-I signaling pathway (PubMed:28469175, PubMed:31006531). |
Cellular Location | Cytoplasm. Cytoplasm, Stress granule |
Tissue Location | Expressed in skeletal muscle, spleen, kidney, placenta, prostate, stomach, thyroid and tongue. Also weakly expressed in heart, thymus, liver and lung. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. This gene is located in a chromosomal region known to be frequently deleted in patients with neurofibromatosis. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq].
References
Zhao, J., et al. BMC Med. Genet. 11, 96 (2010) :
You, F., et al. Nat. Immunol. 10(12):1300-1308(2009)
Visser, R., et al. Am. J. Med. Genet. A 149A (4), 806-808 (2009) :
Oshiumi, H., et al. J. Biol. Chem. 284(2):807-817(2009)
Gao, D., et al. PLoS ONE 4 (6), E5760 (2009) :
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