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HERC3 Antibody(N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q15034 |
| Other Accession | NP_055421.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 117188 Da |
| Antigen Region | 51-77 aa |
| Gene ID | 8916 |
|---|---|
| Other Names | Probable E3 ubiquitin-protein ligase HERC3, 632-, HECT domain and RCC1-like domain-containing protein 3, HERC3, KIAA0032 |
| Target/Specificity | This HERC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-77 amino acids from the N-terminal region of human HERC3. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | HERC3 Antibody(N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | HERC3 |
|---|---|
| Synonyms | KIAA0032 |
| Function | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. |
| Cellular Location | Cytoplasm. Cytoplasmic vesicle. Note=Also found in vesicular-like structures |
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Provided below are standard protocols that you may find useful for product applications.
Background
HERC3 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Hochrainer, K., et al. Genomics 85(2):153-164(2005)
Cruz, C., et al. FEBS Lett. 488 (1-2), 74-80 (2001) :
Cruz, C., et al. Cytogenet. Cell Genet. 87 (3-4), 263-264 (1999) :
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