MCM9 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q9NXL9 |
Other Accession | F1N2W9 |
Reactivity | Human |
Predicted | Bovine |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 127313 Da |
Antigen Region | 316-345 aa |
Gene ID | 254394 |
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Other Names | DNA helicase MCM9, hMCM9, Mini-chromosome maintenance deficient domain-containing protein 1, Minichromosome maintenance 9, MCM9, C6orf61, MCMDC1 |
Target/Specificity | This MCM9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 316-345 amino acids from the C-terminal region of human MCM9. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | MCM9 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | MCM9 |
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Synonyms | C6orf61, MCMDC1 |
Function | Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross- links (ICLs) by homologous recombination (HR) (PubMed:23401855). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity (PubMed:26215093). Probably by regulating the localization of the MRN complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs (PubMed:23401855). Acts as a helicase in DNA mismatch repair (MMR) following DNA replication errors to unwind the mismatch containing DNA strand (PubMed:26300262). In addition, recruits MLH1, a component of the MMR complex, to chromatin (PubMed:26300262). The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression (PubMed:23401855). Probably by regulating HR, plays a key role during gametogenesis (By similarity). |
Cellular Location | Nucleus. Chromosome. Note=Colocalizes to nuclear foci with RPA1 following DNA damage (PubMed:23401855). Localizes to double- stranded DNA breaks (PubMed:23401855). Recruited to chromatin by MSH2 (PubMed:26300262). |
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Provided below are standard protocols that you may find useful for product applications.
Background
MCM9, a member of the MCM2-8 family, binds to chromatin and is required for the recruitment of the MCM2-7 helicase onto chromatin. MCM9 can form a complex with Cdt1. It is thought that MCM9 might play an important role in DNA replication since its depletion results in its inhibition.
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