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INTS9 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - INTS9 Antibody (C-term) AP20367b
    INTS9 Antibody (C-term) (Cat. #AP20367b) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the INTS9 antibody detected the INTS9 protein (arrow).
    detail
  • WB - INTS9 Antibody (C-term) AP20367b
    Anti-INTS9 Antibody (C-term) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 74 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9NV88
Other Accession Q8K114, Q4R5Z4, Q2KJA6
Reactivity Human
Predicted Bovine, Monkey, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 73814 Da
Antigen Region 489-515 aa
Additional Information
Gene ID 55756
Other Names Integrator complex subunit 9, Int9, Protein related to CPSF subunits of 74 kDa, RC-74, INTS9, RC74
Target/Specificity This INTS9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 489-515 amino acids from the C-terminal region of human INTS9.
Dilution WB~~1:1000
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsINTS9 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name INTS9 {ECO:0000303|PubMed:29471365, ECO:0000312|EMBL:BAA91867.1}
Function Component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes (PubMed:25201415, PubMed:33243860, PubMed:33548203, PubMed:38570683). The integrator complex provides a quality checkpoint during transcription elongation by driving premature transcription termination of transcripts that are unfavorably configured for transcriptional elongation: the complex terminates transcription by (1) catalyzing dephosphorylation of the C-terminal domain (CTD) of Pol II subunit POLR2A/RPB1 and SUPT5H/SPT5, (2) degrading the exiting nascent RNA transcript via endonuclease activity and (3) promoting the release of Pol II from bound DNA (PubMed:33243860, PubMed:38570683). The integrator complex is also involved in terminating the synthesis of non-coding Pol II transcripts, such as enhancer RNAs (eRNAs), small nuclear RNAs (snRNAs), telomerase RNAs and long non-coding RNAs (lncRNAs) (PubMed:16239144, PubMed:22252320, PubMed:26308897, PubMed:30737432). Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the integrator complex (PubMed:23904267).
Cellular Location Nucleus. Cytoplasm
Citations (0)
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Background

Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.

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$ 192.50
$ 75.00
Cat# AP20367b
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