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RNF166 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - RNF166 Antibody (Center) AP20484c
    Fluorescent image of NIH/3T3 cells stained with RNF166 Antibody (Center)(Cat#AP20484C). AP20484C was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
    detail
  • WB - RNF166 Antibody (Center) AP20484c
    RNF166 Antibody (Center) (Cat. #AP20484c) western blot analysis in 293,mouse NIH/3T3 cell line and testis tissue lysates (35ug/lane).This demonstrates the RNF166 antibody detected the RNF166 protein (arrow).
    detail
  • SPECIFICATION
  • CITATIONS: 2
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IF, WB, E
Primary Accession Q96A37
Other Accession Q6J1I7, Q3U9F6
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 26122 Da
Antigen Region 71-97 aa
Additional Information
Gene ID 115992
Other Names RING finger protein 166, RNF166
Target/Specificity This RNF166 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-97 amino acids from the Central region of human RNF166.
Dilution IF~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRNF166 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RNF166
Function E3 ubiquitin-protein ligase that promotes the ubiquitination of different substrates (PubMed:27880896). In turn, participates in different biological processes including interferon production or autophagy (PubMed:26456228, PubMed:27880896). Plays a role in the activation of RNA virus-induced interferon-beta production by promoting the ubiquitination of TRAF3 and TRAF6 (PubMed:26456228). Also plays a role in the early recruitment of autophagy adapters to bacteria (PubMed:27880896). Mediates 'Lys-29' and 'Lys-33'-linked ubiquitination of SQSTM1 leading to xenophagic targeting of bacteria and inhibition of their replication (PubMed:27880896).
Cellular Location Cytoplasm.
Research Areas
Citations ( 0 )

References

Ota T., et al. Nat. Genet. 36:40-45(2004).
Mural R.J., et al. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

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$ 365.00
$ 140.00
Cat# AP20484c
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