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PPHLN1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • IF - PPHLN1 Antibody (N-term) AP20529a
    Fluorescent image of Hela cells stained with PPHLN1 Antibody (N-term)(Cat#AP20529A). AP20529A was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • WB - PPHLN1 Antibody (N-term) AP20529a
    PPHLN1 Antibody (N-term) (Cat. #AP20529a) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PPHLN1 antibody detected the PPHLN1 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8NEY8
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 52737 Da
Antigen Region 45-66 aa
Additional Information
Gene ID 51535
Other Names Periphilin-1, Gastric cancer antigen Ga50, PPHLN1
Target/Specificity This PPHLN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-66 amino acids from the N-terminal region of human PPHLN1.
Dilution IF~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPPHLN1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPHLN1 (HGNC:19369)
Function Component of the HUSH complex, a multiprotein complex that mediates epigenetic repression. The HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3. In the HUSH complex, contributes to the maintenance of the complex at chromatin (PubMed:26022416). Acts as a transcriptional corepressor and regulates the cell cycle, probably via the HUSH complex (PubMed:15474462, PubMed:17963697). The HUSH complex is also involved in the silencing of unintegrated retroviral DNA: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed (PubMed:30487602). May be involved in epithelial differentiation by contributing to epidermal integrity and barrier formation (PubMed:12853457).
Cellular Location Nucleus. Cytoplasm. Chromosome. Note=In undifferentiated keratinocytes expressed in speckle-type nuclear granules and at the nuclear membrane, but in the differentiated keratinocytes colocalized with periplakin at the cell periphery and at cell-cell junctions (PubMed:12853457) Localizes to chromatin (PubMed:26022416).
Tissue Location Ubiquitous..
Research Areas
Citations (0)

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Involved in epithelial differentiation and contributes to epidermal integrity and barrier formation.


Imami K., et al. Anal. Sci. 24:161-166(2008).
Line A., et al. Br. J. Cancer 86:1824-1830(2002).
Kazerounian S., et al. J. Biol. Chem. 278:36707-36717(2003).
Zhang Q.-H., et al. Genome Res. 10:1546-1560(2000).
Ota T., et al. Nat. Genet. 36:40-45(2004).

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$ 325.00
$ 109.00
Cat# AP20529a
Availability: In Stock
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