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H2AFY2 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - H2AFY2 Antibody (Center) AP20530c
    H2AFY2 Antibody (Center) (Cat. #AP20530c) western blot analysis in CEM,NCI-H460 cell line lysates (35ug/lane).This demonstrates the H2AFY2 antibody detected the H2AFY2 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9P0M6
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 148-169 aa
Additional Information
Other Names Core histone macro-H2A2, Histone macroH2A2, mH2A2, H2AFY2, MACROH2A2
Target/Specificity This H2AFY2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 148-169 amino acids from the Central region of human H2AFY2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsH2AFY2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MACROH2A2 (HGNC:14453)
Function Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.
Cellular Location Nucleus. Chromosome. Note=Enriched in inactive X chromosome chromatin (PubMed:11331621, PubMed:11262398) and in senescence- associated heterochromatin (PubMed:15621527) EMBL; AF336304; AAK52471.1; -; mRNA EMBL; AF151534; AAF72101.1; -; mRNA EMBL; AK022776; BAB14239.1; -; mRNA EMBL; AL731540; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; BC016172; AAH16172.1; -; mRNA CCDS; CCDS7296.1; - RefSeq; NP_061119.1; NM_018649.2 PDB; 2XD7; X-ray; 2.09 A; A/B/C/D=177-369 PDB; 6FY5; X-ray; 1.65 A; A/B=178-372 PDBsum; 2XD7; - PDBsum; 6FY5; - SMR; Q9P0M6; - BioGRID; 120686; 129 DIP; DIP-48563N; - IntAct; Q9P0M6; 102 MINT; Q9P0M6; - STRING; 9606.ENSP00000362352; - iPTMnet; Q9P0M6; - PhosphoSitePlus; Q9P0M6; - BioMuta; H2AFY2; - DMDM; 12585260; - EPD; Q9P0M6; - jPOST; Q9P0M6; - MassIVE; Q9P0M6; - MaxQB; Q9P0M6; - PaxDb; Q9P0M6; - PeptideAtlas; Q9P0M6; - PRIDE; Q9P0M6; - ProteomicsDB; 83580; - Antibodypedia; 28963; 325 antibodies DNASU; 55506; - Ensembl; ENST00000373255; ENSP00000362352; ENSG00000099284 GeneID; 55506; - KEGG; hsa:55506; - UCSC; uc001jqm.4; human CTD; 55506; - DisGeNET; 55506; - EuPathDB; HostDB:ENSG00000099284.13; - GeneCards; MACROH2A2; - HGNC; HGNC:14453; MACROH2A2 HPA; ENSG00000099284; Low tissue specificity MIM; 616141; gene neXtProt; NX_Q9P0M6; - OpenTargets; ENSG00000099284; - PharmGKB; PA29118; - eggNOG; KOG1756; Eukaryota eggNOG; KOG2633; Eukaryota GeneTree; ENSGT00940000158120; - InParanoid; Q9P0M6; - KO; K11251; - OMA; GRKSMVN; - OrthoDB; 1504122at2759; - PhylomeDB; Q9P0M6; - TreeFam; TF332276; - PathwayCommons; Q9P0M6; - BioGRID-ORCS; 55506; 3 hits in 876 CRISPR screens ChiTaRS; H2AFY2; human EvolutionaryTrace; Q9P0M6; - GeneWiki; H2AFY2; - GenomeRNAi; 55506; - Pharos; Q9P0M6; Tbio PRO; PR:Q9P0M6; - Proteomes; UP000005640; Chromosome 10 RNAct; Q9P0M6; protein Bgee; ENSG00000099284; Expressed in embryo and 239 other tissues ExpressionAtlas; Q9P0M6; baseline and differential Genevisible; Q9P0M6; HS GO; GO:0001740; C:Barr body; IDA:MGI GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB GO; GO:0000790; C:nuclear chromatin; IDA:UniProtKB GO; GO:0000784; C:nuclear chromosome, telomeric region; HDA:BHF-UCL GO; GO:0005654; C:nucleoplasm; IDA:HPA GO; GO:0000786; C:nucleosome; IEA:UniProtKB-KW GO; GO:0031490; F:chromatin DNA binding; IDA:UniProtKB GO; GO:0003677; F:DNA binding; IBA:GO_Central GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:UniProtKB GO; GO:0000976; F:transcription regulatory region sequence-specific DNA binding; IDA:UniProtKB GO; GO:0007420; P:brain development; IMP:UniProtKB GO; GO:0006325; P:chromatin organization; IBA:GO_Central GO; GO:0006342; P:chromatin silencing; IBA:GO_Central GO; GO:0007549; P:dosage compensation; IDA:MGI GO; GO:0071169; P:establishment of protein localization to chromatin; IMP:UniProtKB GO; GO:0045814; P:negative regulation of gene expression, epigenetic; IMP:UniProtKB GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:UniProtKB GO; GO:1901837; P:negative regulation of transcription of nucleolar large rRNA by RNA polymerase I; IGI:UniProtKB GO; GO:0006334; P:nucleosome assembly; IEA:InterPro GO; GO:0045618; P:positive regulation of keratinocyte differentiation; IMP:UniProtKB CDD; cd00074; H2A; 1 CDD; cd02904; Macro_H2A_like; 1 Gene3D; 1.10.20.10; -; 1 Gene3D; 3.40.220.10; -; 1 InterPro; IPR021171; Core_histone_macro-H2A InterPro; IPR009072; Histone-fold InterPro; IPR002119; Histone_H2A InterPro; IPR007125; Histone_H2A/H2B/H3 InterPro; IPR032454; Histone_H2A_C InterPro; IPR002589; Macro_dom InterPro; IPR043472; Macro_dom-like InterPro; IPR035796; Macro_H2A Pfam; PF00125; Histone; 1 Pfam; PF16211; Histone_H2A_C; 1 Pfam; PF01661; Macro; 1 PIRSF; PIRSF037942; Core_histone_macro-H2A; 1 PRINTS; PR00620; HISTONEH2A SMART; SM00506; A1pp; 1 SMART; SM00414; H2A; 1 SUPFAM; SSF47113; SSF47113; 1 SUPFAM; SSF52949; SSF52949; 1 PROSITE; PS51154; MACRO; 1 1: Evidence at protein level; 3D-structure; Chromatin regulator; Chromosome; DNA-binding; Isopeptide bond; Nucleosome core; Nucleus; Reference proteome; Ubl conjugation CHAIN 1..372 /note="Core histone macro-H2A.2" /id="PRO_0000055320" DOMAIN 2..117 /note="Histone H2A" DOMAIN 184..370 /note="Macro" /evidence="ECO:0000255|PROSITE-ProRule:PRU00490" COMPBIAS 118..161 /note="Lys-rich" CROSSLNK 239 /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" /evidence="ECO:0000244|PubMed:25755297, ECO:0000244|PubMed:28112733" STRAND 183..191 /evidence="ECO:0000244|PDB:6FY5" STRAND 196..202 /evidence="ECO:0000244|PDB:6FY5" HELIX 204..209 /evidence="ECO:0000244|PDB:6FY5" STRAND 213..219 /evidence="ECO:0000244|PDB:6FY5" HELIX 227..252 /evidence="ECO:0000244|PDB:6FY5" STRAND 260..264 /evidence="ECO:0000244|PDB:6FY5" STRAND 266..277 /evidence="ECO:0000244|PDB:6FY5" HELIX 286..302 /evidence="ECO:0000244|PDB:6FY5" TURN 303..305 /evidence="ECO:0000244|PDB:6FY5" STRAND 307..311 /evidence="ECO:0000244|PDB:6FY5" HELIX 323..339 /evidence="ECO:0000244|PDB:6FY5" STRAND 348..353 /evidence="ECO:0000244|PDB:6FY5" HELIX 356..366 /evidence="ECO:0000244|PDB:6FY5" STRAND 369..371 /evidence="ECO:0000244|PDB:6FY5" SEQUENCE 372 AA; 40058 MW; C4B35CD156031AF1 CRC64; MSGRSGKKKM SKLSRSARAG VIFPVGRLMR YLKKGTFKYR ISVGAPVYMA AVIEYLAAEI LELAGNAARD NKKARIAPRH ILLAVANDEE LNQLLKGVTI ASGGVLPRIH PELLAKKRGT KGKSETILSP PPEKRGRKAT SGKKGGKKSK AAKPRTSKKS KPKDSDKEGT SNSTSEDGPG DGFTILSSKS LVLGQKLSLT QSDISHIGSM RVEGIVHPTT AEIDLKEDIG KALEKAGGKE FLETVKELRK SQGPLEVAEA AVSQSSGLAA KFVIHCHIPQ WGSDKCEEQL EETIKNCLSA AEDKKLKSVA FPPFPSGRNC FPKQTAAQVT LKAISAHFDD SSASSLKNVY FLLFDSESIG IYVQEMAKLD AK
Research Areas
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Background

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.

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$ 325.00
$ 109.00
Cat# AP20530c
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