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ALDH1A1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - ALDH1A1 Antibody (Center) AP20580c
    Fluorescent image of A549 cells stained with ALDH1A1 Antibody (Center)(Cat#AP20580c). AP20580c was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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  • WB - ALDH1A1 Antibody (Center) AP20580c
    All lanes : Anti-ALDH1A1 Antibody (Center) at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: HT-29 whole cell lysate Lane 4: Mouse lung lysate Lane 5: Mouse liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P-Leica - ALDH1A1 Antibody (Center) AP20580c
    Immunohistochemical analysis of paraffin-embedded Human stomach tissue using AP20580c performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • IHC-P-Leica - ALDH1A1 Antibody (Center) AP20580c
    Immunohistochemical analysis of paraffin-embedded Human testis tissue using AP20580c performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P-Leica, IF, WB, E
Primary Accession P00352
Other Accession Q8HYE4
Reactivity Human, Mouse, Rat
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 54862 Da
Additional Information
Gene ID 216
Other Names Retinal dehydrogenase 1, RALDH 1, RalDH1, ALDH-E1, ALHDII, Aldehyde dehydrogenase family 1 member A1, Aldehyde dehydrogenase, cytosolic, ALDH1A1, ALDC, ALDH1, PUMB1
Target/Specificity This ALDH1A1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 306-339 amino acids from the Central region of human ALDH1A1.
Dilution IF~~1:25
WB~~1:1000
IHC-P-Leica~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALDH1A1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ALDH1A1 (HGNC:402)
Function Cytosolic dehydrogenase that catalyzes the irreversible oxidation of a wide range of aldehydes to their corresponding carboxylic acid (PubMed:19296407, PubMed:12941160, PubMed:15623782, PubMed:17175089, PubMed:26373694, PubMed:25450233). Functions downstream of retinol dehydrogenases and catalyzes the oxidation of retinaldehyde into retinoic acid, the second step in the oxidation of retinol/vitamin A into retinoic acid (By similarity). This pathway is crucial to control the levels of retinol and retinoic acid, two important molecules which excess can be teratogenic and cytotoxic (By similarity). Also oxidizes aldehydes resulting from lipid peroxidation like (E)-4-hydroxynon-2-enal/HNE, malonaldehyde and hexanal that form protein adducts and are highly cytotoxic. By participating for instance to the clearance of (E)-4-hydroxynon-2-enal/HNE in the lens epithelium prevents the formation of HNE-protein adducts and lens opacification (PubMed:19296407, PubMed:12941160, PubMed:15623782). Functions also downstream of fructosamine-3-kinase in the fructosamine degradation pathway by catalyzing the oxidation of 3-deoxyglucosone, the carbohydrate product of fructosamine 3-phosphate decomposition, which is itself a potent glycating agent that may react with lysine and arginine side-chains of proteins (PubMed:17175089). Has also an aminobutyraldehyde dehydrogenase activity and is probably part of an alternative pathway for the biosynthesis of GABA/4-aminobutanoate in midbrain, thereby playing a role in GABAergic synaptic transmission (By similarity).
Cellular Location Cytoplasm, cytosol. Cell projection, axon {ECO:0000250|UniProtKB:P24549}
Tissue Location Expressed by erythrocytes (at protein level).
Research Areas
Citations (0)
citation

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Background

Binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid (By similarity).

References

Hsu L.C.,et al.Genomics 5:857-865(1989).
Zheng C.F.,et al.Alcohol. Clin. Exp. Res. 17:828-831(1993).
Ramana K.V.,et al.Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Humphray S.J.,et al.Nature 429:369-374(2004).

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$ 365.00
$ 140.00
Cat# AP20580c
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