|Application ||WB, E|
|Calculated MW||23510 Da|
|Other Names||Mitotic spindle assembly checkpoint protein MAD2A, HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1, MAD2L1, MAD2|
|Target/Specificity||This MAD2L1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 159-192 amino acids from the C-terminal region of human MAD2L1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MAD2L1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.|
|Cellular Location||Nucleus. Chromosome, centromere, kinetochore. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Note=Recruited by MAD1L1 to unattached kinetochores (Probable). Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD.|
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Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore- spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.
Li Y.,et al.Science 274:246-248(1996).
Gemma A.,et al.Lung Cancer 32:289-295(2001).
Jin D.-Y.,et al.Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases.
Klebert S.,et al.Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
Nobori T.,et al.Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
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