ZNRF2 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q8NHG8 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 24115 Da |
Gene ID | 223082 |
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Other Names | E3 ubiquitin-protein ligase ZNRF2, 632-, Protein Ells2, RING finger protein 202, Zinc/RING finger protein 2, ZNRF2, RNF202 |
Target/Specificity | This ZNRF2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 2-36 amino acids from the N-terminal region of human ZNRF2. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | ZNRF2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ZNRF2 |
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Synonyms | RNF202 |
Function | E3 ubiquitin-protein ligase that plays a role in the establishment and maintenance of neuronal transmission and plasticity. Ubiquitinates the Na(+)/K(+) ATPase alpha-1 subunit/ATP1A1 and thereby influences its endocytosis and/or degradation (PubMed:22797923). Acts also as a positive regulator of mTORC1 activation by amino acids, which functions upstream of the V-ATPase and of Rag-GTPases (PubMed:27244671). In turn, phosphorylation by mTOR leads to its inhibition via targeting to the cytosol allowing a self-regulating feedback mechanism (PubMed:27244671). |
Cellular Location | Endosome membrane; Peripheral membrane protein. Lysosome membrane; Peripheral membrane protein. Presynaptic cell membrane; Peripheral membrane protein. Cytoplasm |
Tissue Location | Highly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney. |
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Provided below are standard protocols that you may find useful for product applications.
Background
May play a role in the establishment and maintenance of neuronal transmission and plasticity via its ubiquitin ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
References
Araki T.,et al.J. Neurosci. 23:9385-9394(2003).
Guo J.H.,et al.Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
Olsen J.V.,et al.Cell 127:635-648(2006).
Plans V.,et al.J. Cell. Biochem. 97:572-582(2006).
Cantin G.T.,et al.J. Proteome Res. 7:1346-1351(2008).
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