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CHRNB3 Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q05901 |
| Other Accession | P12391, Q8BMN3 |
| Reactivity | Hamster |
| Predicted | Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 52729 Da |
| Antigen Region | 187-200 aa |
| Gene ID | 1142 |
|---|---|
| Other Names | Neuronal acetylcholine receptor subunit beta-3, CHRNB3 |
| Target/Specificity | This CHRNB3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 187-200 amino acids from the Central region of human CHRNB3. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CHRNB3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CHRNB3 (HGNC:1963) |
|---|---|
| Function | Component of neuronal acetylcholine receptors (nAChRs) that function as pentameric, ligand-gated cation channels with high calcium permeability among other activities. nAChRs are excitatory neurotrasnmitter receptors formed by a collection of nAChR subunits known to mediate synaptic transmission in the nervous system and the neuromuscular junction. Each nAchR subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, cation permeability, and binding to allosteric modulators (PubMed:11118490, PubMed:16835356, PubMed:35889515). Has an accessory rather than functional role and is only able to form functional nAChRs when co-assembled with another beta subunit (PubMed:11118490, PubMed:16835356). Participates in pentameric assemblies along with CHRNA3, CHRNA4, CHRNA6, CHRNB2 and CHRNB4 (PubMed:11118490, PubMed:16835356). Modulates receptor assembly and increases receptor sensitivity to nicotine when associated with CHRNB2, CHRNA4 and/or CHRNA6 as well as CHRNA3 and CHRNB4 (PubMed:11118490, PubMed:16835356). Seems to play a role in nicotine addiction (PubMed:11118490, PubMed:16835356). |
| Cellular Location | Synaptic cell membrane {ECO:0000250|UniProtKB:O70174}; Multi-pass membrane protein. Cell membrane {ECO:0000250|UniProtKB:O70174}; Multi-pass membrane protein |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
References
Elliott K.J.,et al.J. Mol. Neurosci. 7:217-228(1996).
Groot Kormelink P.J.,et al.FEBS Lett. 400:309-314(1997).
Keddache M.,et al.Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
Willoughby J.J.,et al.Neurosci. Lett. 155:136-139(1993).
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