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NCAM1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NCAM1 Antibody (C-term) AP20927c
    All lanes : Anti-NCAM1 Antibody (C-term) at 1:500 dilution Lane 1: Human brain lysate Lane 2: Mouse brain lysate Lane 3: Rat brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 95 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P-Leica - NCAM1 Antibody (C-term) AP20927c
    Immunohistochemical analysis of paraffin-embedded Human brain tissue using AP20927c performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • IHC-P-Leica - NCAM1 Antibody (C-term) AP20927c
    Immunohistochemical analysis of paraffin-embedded Human brain tissue using AP20927c performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • IHC-P-Leica - NCAM1 Antibody (C-term) AP20927c
    Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue using AP20927c performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P-Leica, WB, E
Primary Accession P13591
Other Accession P13596, P13595, P13590, P31836
Reactivity Human, Mouse, Rat
Predicted Bovine, Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 94574 Da
Additional Information
Gene ID 4684
Other Names Neural cell adhesion molecule 1, N-CAM-1, NCAM-1, CD56, NCAM1, NCAM
Target/Specificity This NCAM1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 796-830 amino acids from the C-terminal region of human NCAM1.
Dilution WB~~1:500
IHC-P-Leica~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNCAM1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NCAM1 (HGNC:7656)
Synonyms NCAM
Function This protein is a cell adhesion molecule involved in neuron- neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. (Microbial infection) Acts as a receptor for Zika virus.
Cellular Location [Isoform 1]: Cell membrane; Single-pass type I membrane protein [Isoform 3]: Cell membrane; Lipid-anchor, GPI- anchor [Isoform 5]: Secreted.
Research Areas
Citations (0)
citation

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Background

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

References

Barton C.H.,et al.Development 104:165-173(1988).
Lanier L.L.,et al.J. Immunol. 146:4421-4426(1991).
Saito S.,et al.Lung Cancer 10:307-318(1994).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.

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$ 182.50
$ 70.00
Cat# AP20927c
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