TRIAD3 (UBCE7IP1) Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, E |
---|---|
Primary Accession | Q9NWF9 |
Other Accession | P58283 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 99406 Da |
Antigen Region | 716-745 aa |
Gene ID | 54476 |
---|---|
Other Names | E3 ubiquitin-protein ligase RNF216, 632-, RING finger protein 216, Triad domain-containing protein 3, Ubiquitin-conjugating enzyme 7-interacting protein 1, Zinc finger protein inhibiting NF-kappa-B, RNF216, TRIAD3, UBCE7IP1, ZIN |
Target/Specificity | This TRIAD3 (UBCE7IP1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 716-745 amino acids from the C-terminal region of human TRIAD3 (UBCE7IP1). |
Dilution | IHC-P~~1:50~100 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | TRIAD3 (UBCE7IP1) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | RNF216 |
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Synonyms | TRIAD3, UBCE7IP1, ZIN |
Function | [Isoform 1]: E3 ubiquitin ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their ubiquitination (PubMed:34998453). Plays a role in the regulation of antiviral responses by promoting the degradation of TRAF3, TLR4 and TLR9 (PubMed:15107846, PubMed:19893624). In turn, down-regulates NF-kappa-B and IRF3 activation as well as beta interferon production. Participates also in the regulation of autophagy by ubiquitinating BECN1 leading to its degradation and autophagy inhibition (PubMed:25484083). Plays a role in ARC-dependent synaptic plasticity by mediating ARC ubiquitination resulting in its rapid proteasomal degradation (PubMed:24945773). Plays aso an essential role in spermatogenesis and male fertility (By similarity). Mechanistically, regulates meiosis by promoting the degradation of PRKACB through the ubiquitin-mediated lysosome pathway (By similarity). Modulates the gonadotropin-releasing hormone signal pathway by affecting the stability of STAU2 that is required for the microtubule-dependent transport of neuronal RNA from the cell body to the dendrite (By similarity). |
Cellular Location | Cytoplasm. Cytoplasmic vesicle, clathrin-coated vesicle |
Tissue Location | Ubiquitous, with the highest levels of expression in testis and peripheral blood leukocytes |
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Provided below are standard protocols that you may find useful for product applications.
Background
UBCE7IP1 is a cytoplasmic protein which specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains of the encoded protein are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. The encoded protein may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates.
References
Ota, T., et al., Nat. Genet. 36(1):40-45 (2004).
Chen, D., et al., J. Biol. Chem. 277(18):15985-15991 (2002).
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
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