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DKK1 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - DKK1 Antibody AP21147a
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized U-251 MG cells labeling DKK1 with AP21147a at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Cytoplasm and Weak Nucleus staining on U-251 MG cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin(red). The nuclear counter stain is DAPI (blue).
    detail
  • WB - DKK1 Antibody AP21147a
    Anti-DKK1 Antibody at 1:1000 dilution + HepG2 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - DKK1 Antibody AP21147a
    All lanes : Anti-DKK1 Antibody at 1:2000 dilution Lane 1: A549 whole cell lysates Lane 2: Jurkat whole cell lysates Lane 3: NIH/3T3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - DKK1 Antibody AP21147a
    Anti-DKK1 Antibody at 1:4000 dilution + Hela whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - DKK1 Antibody AP21147a
    All lanes : Anti-DKK1 Antibody at 1:2000 dilution Lane 1: Mouse brain lysate Lane 2: Rat brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P-Leica - DKK1 Antibody AP21147a
    Immunohistochemical analysis of paraffin-embedded Human placenta tissue using AP21147a performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • IHC-P-Leica - DKK1 Antibody AP21147a
    Immunohistochemical analysis of paraffin-embedded Human uterus tissue using AP21147a performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P-Leica, IF, WB, E
Primary Accession O94907
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 28672 Da
Antigen Region 1-266 aa
Additional Information
Gene ID 22943
Other Names Dickkopf-related protein 1, Dickkopf-1, Dkk-1, hDkk-1, SK, DKK1
Target/Specificity This DKK1 antibody is generated from a rabbit immunized with a recombinant protein of human DKK1.
Dilution IF~~1:25
WB~~1:2000
IHC-P-Leica~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDKK1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DKK1
Function Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed:22000856). DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (PubMed:17143291). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (By similarity).
Cellular Location Secreted.
Tissue Location Placenta.
Research Areas
Citations (0)
citation

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Background

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

References

Fedi P.,et al.J. Biol. Chem. 274:19465-19472(1999).
Krupnik V.E.,et al.Gene 238:301-313(1999).
Tate G.,et al.Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases.
Roessler E.,et al.Cytogenet. Cell Genet. 89:220-224(2000).
Clark H.F.,et al.Genome Res. 13:2265-2270(2003).

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$ 182.50
$ 70.00
Cat# AP21147a
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