(Mouse) Rybp Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q8CCI5 |
Reactivity | Mouse |
Host | Rabbit |
Clonality | polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 24777 Da |
Gene ID | 56353 |
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Other Names | RING1 and YY1-binding protein, Death effector domain-associated factor, DED-associated factor, Rybp, Dedaf |
Target/Specificity | This Mouse Rybp antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 131-165 amino acids from the Central region of Mouse Rybp. |
Dilution | WB~~1:2000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | (Mouse) Rybp Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Rybp |
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Synonyms | Dedaf |
Function | Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1-like complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:22325148, PubMed:28596365). Component of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females (PubMed:28596365). May stimulate ubiquitination of histone H2A 'Lys-119' by recruiting the complex to target sites (PubMed:22325148, PubMed:28596365). Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes (By similarity). May also regulate the ubiquitin-mediated proteasomal degradation of other proteins like FANK1 to regulate apoptosis (PubMed:17874297). May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1 (By similarity). May bind to DNA (PubMed:19170609). May play a role in the repression of tumor growth and metastasis in breast cancer by down-regulating SRRM3 (PubMed:27748911). |
Cellular Location | Nucleus. Cytoplasm {ECO:0000250|UniProtKB:Q8N488}. Nucleus, nucleoplasm. Note=Primarily found in the nucleus Detected in a punctate pattern likely to represent Polycomb group (PcG) bodies. |
Tissue Location | Expressed in embryonic stem cells. |
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Background
Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. May bind to DNA. Promotes apoptosis (By similarity).
References
Carninci P.,et al.Science 309:1559-1563(2005).
Garcia E.,et al.EMBO J. 18:3404-3418(1999).
Danen-van Oorschot A.A.M.M.,et al.Cell Death Differ. 11:564-573(2004).
Pirity M.K.,et al.Mol. Cell. Biol. 25:7193-7202(2005).
Arrigoni R.,et al.FEBS Lett. 580:6233-6241(2006).
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