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PNMT Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PNMT Antibody (Center) AP21394c
    Anti-PNMT Antibody (Center)at 1:2000 dilution + SW620 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P11086
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 30855 Da
Additional Information
Gene ID 5409
Other Names Phenylethanolamine N-methyltransferase, PNMTase, Noradrenaline N-methyltransferase, PNMT, PENT
Target/Specificity This PNMT antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 158-192 amino acids from the Central region of human PNMT.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPNMT Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PNMT
Synonyms PENT
Function Catalyzes the transmethylation of nonepinephrine (noradrenaline) to form epinephrine (adrenaline), using S-adenosyl-L- methionine as the methyl donor (PubMed:20496117). Other substrates include phenylethanolamine and octopamine (PubMed:8812853, PubMed:16363801, PubMed:16277617). Also methylates normetanephrine (By similarity).
Research Areas
Citations (0)
citation

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Background

Converts noradrenaline to adrenaline.

References

Kaneda N.,et al.J. Biol. Chem. 263:7672-7677(1988).
Sasaoka T.,et al.Neurochem. Int. 15:555-565(1989).
Baetge E.E.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:3648-3652(1988).
Martin J.L.,et al.Structure 9:977-985(2001).
Gee C.L.,et al.Biochemistry 44:16875-16885(2005).

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$ 182.50
$ 70.00
Cat# AP21394c
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