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USP29 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | Q9HBJ7 |
| Other Accession | NP_065954 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 104156 Da |
| Antigen Region | 660-690 aa |
| Gene ID | 57663 |
|---|---|
| Other Names | Ubiquitin carboxyl-terminal hydrolase 29, Deubiquitinating enzyme 29, Ubiquitin thioesterase 29, Ubiquitin-specific-processing protease 29, USP29 |
| Target/Specificity | This USP29 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 660-690 amino acids from the Central region of human USP29. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | USP29 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | USP29 {ECO:0000303|PubMed:10958632, ECO:0000312|HGNC:HGNC:18563} |
|---|---|
| Function | Deubiquitinase involved in innate antiviral immunity by mediating 'Lys-48'-linked deubiquitination of CGAS, thereby promoting its stabilization. |
| Cellular Location | Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:Q9ES63}. Note=Localizes to perinuclear region in response to herpes simplex virus-1 (HSV-1) infection {ECO:0000250|UniProtKB:Q9ES63} |
Provided below are standard protocols that you may find useful for product applications.
Background
Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.
References
Puente, X.S., et al., Nat. Rev. Genet. 4(7):544-558 (2003).
Tureci, O., et al., Oncogene 21(24):3879-3888 (2002).
Kim, J., et al., Genome Res. 10(8):1138-1147 (2000).
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