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RAP1GAP Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RAP1GAP Antibody (N-Term) AP21784a
    Anti-RAP1GAP Antibody (N-Term) at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P47736
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 73361 Da
Additional Information
Gene ID 5909
Other Names Rap1 GTPase-activating protein 1, Rap1GAP, Rap1GAP1, RAP1GAP, KIAA0474, RAP1GA1
Target/Specificity This RAP1GAP antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 105-128 amino acids from human RAP1GAP.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAP1GAP Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAP1GAP
Synonyms KIAA0474, RAP1GA1
Function GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP- bound state.
Cellular Location Golgi apparatus membrane; Peripheral membrane protein
Tissue Location Significant expression seen in the brain, kidney and pancreas. Abundant in the cerebral cortex and expressed at much lower levels in the spinal cord. Not detected in the lymphoid tissues
Research Areas
Citations (0)
citation

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Background

GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.

References

Rubinfeld B.,et al.Cell 65:1033-1042(1991).
Seki N.,et al.DNA Res. 4:345-349(1997).
Gregory S.G.,et al.Nature 441:315-321(2006).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Mochizuki N.,et al.Nature 400:891-894(1999).

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$ 182.50
$ 70.00
Cat# AP21784a
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