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HUMAN-LDHB(Y240)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HUMAN-LDHB(Y240) AP21816a
    Anti-LDHB Antibody (Y240) at 1:2000 dilution + human heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - HUMAN-LDHB(Y240) AP21816a
    All lanes : Anti-HUMAN-LDHB(Y240) at 1:2000 dilution Lane 1: Mouse heart whole cell lysate Lane 2: Mouse brain whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • SPECIFICATION
  • CITATIONS: 1
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P07195
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 36638 Da
Additional Information
Gene ID 3945
Other Names L-lactate dehydrogenase B chain, LDH-B, LDH heart subunit, LDH-H, Renal carcinoma antigen NY-REN-46, LDHB
Target/Specificity This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 220-250 amino acids of human.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHUMAN-LDHB(Y240) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LDHB
Function Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).
Cellular Location Cytoplasm. Mitochondrion inner membrane; Peripheral membrane protein
Tissue Location Predominantly expressed in aerobic tissues such as cardiac muscle.
Research Areas
Citations ( 0 )

References

Takeno T.,et al.Biochem. J. 257:921-924(1989).
Sakai I.,et al.Biochem. J. 248:933-936(1987).
Bienvenut W.V.,et al.Submitted (MAY-2005) to UniProtKB.
Lubec G.,et al.Submitted (DEC-2008) to UniProtKB.
Scanlan M.J.,et al.Int. J. Cancer 83:456-464(1999).

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$ 182.50
$ 70.00
Cat# AP21816a
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