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DDX42 Antibody (N-Term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q86XP3 |
| Other Accession | Q810A7, Q5R7D1 |
| Reactivity | Human, Mouse |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 102975 Da |
| Gene ID | 11325 |
|---|---|
| Other Names | ATP-dependent RNA helicase DDX42, DEAD box protein 42, RNA helicase-like protein, RHELP, RNA helicase-related protein, RNAHP, SF3b DEAD box protein, Splicing factor 3B-associated 125 kDa protein, SF3b125, DDX42 |
| Target/Specificity | This DDX42 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 104-138 amino acids from human DDX42. |
| Dilution | WB~~1:2000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | DDX42 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | DDX42 {ECO:0000303|PubMed:16397294, ECO:0000312|HGNC:HGNC:18676} |
|---|---|
| Function | ATP-dependent RNA helicase that binds to partially double- stranded RNAs (dsRNAs) in order to unwind RNA secondary structures (PubMed:16397294). Unwinding is promoted in the presence of single- strand binding proteins (PubMed:16397294). Mediates also RNA duplex formation thereby displacing the single-strand RNA binding protein (PubMed:16397294). ATP and ADP modulate its activity: ATP binding and hydrolysis by DDX42 triggers RNA strand separation, whereas the ADP- bound form of the protein triggers annealing of complementary RNA strands (PubMed:16397294). Required for assembly of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs: DDX42 associates transiently with the SF3B subcomplex of the 17S U2 SnRNP complex and is released after fulfilling its role in the assembly of 17S U2 SnRNP (PubMed:12234937, PubMed:36797247). Involved in the survival of cells by interacting with TP53BP2 and thereby counteracting the apoptosis- stimulating activity of TP53BP2 (PubMed:19377511). Relocalizes TP53BP2 to the cytoplasm (PubMed:19377511). |
| Cellular Location | Cytoplasm. Nucleus |
| Tissue Location | Expressed in several cell lines (at protein level). Expressed in liver, lung, tonsil, thymus, muscle and pancreatic islets |
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Provided below are standard protocols that you may find useful for product applications.
Background
ATP-dependent RNA helicase. Binds to partially double- stranded RNAs (dsRNAs) in order to unwind RNA secondary structures. Unwinding is promoted in the presence of single-strand binding proteins. Mediates also RNA duplex formation thereby displacing the single-strand RNA binding protein. ATP and ADP modulate its activity: ATP binding and hydrolysis by DDX42 triggers RNA strand separation, whereas the ADP-bound form of the protein triggers annealing of complementary RNA strands. Involved in the survival of cells by interacting with TP53BP2 and thereby counteracting the apoptosis-stimulating activity of TP53BP2. Relocalizes TP53BP2 to the cytoplasm.
References
Suk K.,et al.Biochim. Biophys. Acta 1501:63-69(2000).
Ikeda A.,et al.Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Zody M.C.,et al.Nature 440:1045-1049(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
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