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RAD54B Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - RAD54B Antibody (N-Term) AP21946a
    All lanes : Anti-RAD54B Antibody (N-Term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: MOLT-4 whole cell lysate Lane 4: CCRF-CEM whole cell lysate Lane 5: DU145 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 103 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9Y620
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 102967 Da
Additional Information
Gene ID 25788
Other Names DNA repair and recombination protein RAD54B, 3.6.4.-, RAD54 homolog B, RAD54B
Target/Specificity This RAD54B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 111-142 amino acids from human RAD54B.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAD54B Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAD54B
Function Involved in DNA repair and mitotic recombination. May play an active role in recombination processes in concert with other members of the RAD52 epistasis group.
Cellular Location Nucleus.
Tissue Location Abundantly expressed in testis and spleen. Relatively low levels observed in thymus, prostate, ovary and colon
Research Areas
Citations (0)
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Background

Involved in DNA repair and mitotic recombination. May play an active role in recombination processes in concert with other members of the RAD52 epistasis group.

References

Hiramoto T.,et al.Oncogene 18:3422-3426(1999).
Li W.B.,et al.Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
Nusbaum C.,et al.Nature 439:331-335(2006).
Tanaka K.,et al.J. Biol. Chem. 275:26316-26321(2000).
Miyagawa K.,et al.EMBO J. 21:175-180(2002).

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$ 365.00
$ 140.00
Cat# AP21946a
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