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DNAJC3 Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, FC, E |
|---|---|
| Primary Accession | Q13217 |
| Other Accession | Q9R0T3 |
| Reactivity | Human, Mouse |
| Predicted | Rat |
| Host | Rabbit |
| Clonality | polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 57580 Da |
| Gene ID | 5611 |
|---|---|
| Other Names | DnaJ homolog subfamily C member 3, Endoplasmic reticulum DNA J domain-containing protein 6, ER-resident protein ERdj6, ERdj6, Interferon-induced, double-stranded RNA-activated protein kinase inhibitor, Protein kinase inhibitor of 58 kDa, Protein kinase inhibitor p58, DNAJC3, P58IPK, PRKRI |
| Target/Specificity | This DNAJC3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 189-223 amino acids of human DNAJC3. |
| Dilution | WB~~1:2000 FC~~1:25 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | DNAJC3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | DNAJC3 |
|---|---|
| Synonyms | P58IPK, PRKRI |
| Function | Involved in the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Acts as a negative regulator of the EIF2AK4/GCN2 kinase activity by preventing the phosphorylation of eIF- 2-alpha at 'Ser-52' and hence attenuating general protein synthesis under ER stress, hypothermic and amino acid starving stress conditions (By similarity). Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity. |
| Cellular Location | Endoplasmic reticulum. |
| Tissue Location | Widely expressed with high level in the pancreas and testis. Also expressed in cell lines with different levels |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Involved in the unfolded protein response (UPR) during ER stress. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity.
References
Korth M.J.,et al.Gene 170:181-188(1996).
Dunham A.,et al.Nature 428:522-528(2004).
Polyak S.J.,et al.J. Biol. Chem. 271:1702-1707(1996).
Gale M.J. Jr.,et al.Mol. Cell. Biol. 18:859-871(1998).
Melville M.W.,et al.J. Biol. Chem. 274:3797-3803(1999).
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